AP4A Hydrolase Antibody (F-5): sc-271410
- AP4A Hydrolase Antibody (F-5) is a mouse monoclonal IgG1 κ AP4A Hydrolase antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-147 representing full length AP4A Hydrolase of human origin
- AP4A Hydrolase Antibody (F-5) is recommended for detection of AP4A Hydrolase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-AP4A Hydrolase Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AP4A Hydrolase (F-5): sc-271410.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AP4A Hydrolase Antibody (F-5) for WB and IHC(P) applications. These reagents are now offered in bundles with AP4A Hydrolase Antibody (F-5) (see ordering information below).
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AP4A Hydrolase Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects AP4A Hydrolase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). AP4A Hydrolase (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AP4A hydrolase plays a crucial role in cellular metabolism by hydrolyzing asymmetric diadenosine 5′,5′′′-P(1) P(4)-tetraphosphate (Ap4A) into ATP and AMP, thus maintaining nucleotide homeostasis. This enzymatic activity is particularly important in pancreatic beta cells, where AP4A hydrolase regulates ATP-sensitive potassium channels, leading to membrane depolarization and subsequent insulin secretion. Dysregulation of this process may contribute to the pathophysiology of diabetes mellitus, as elevated Ap4A levels can result in increased blood glucose and decreased plasma insulin. Additionally, AP4A hydrolase exhibits activity towards other adenosine and diadenosine polyphosphates with four or more phosphate groups, further underscoring its significance in cellular stress responses and metabolic regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AP4A Hydrolase Antibody (F-5) References:
- Cloning, characterisation and crystallisation of a diadenosine 5',5''-P(1),P(4)-tetraphosphate pyrophosphohydrolase from Caenorhabditis elegans. | Abdelghany, HM., et al. 2001. Biochim Biophys Acta. 1550: 27-36. PMID: 11738085
- The structure of Ap(4)A hydrolase complexed with ATP-MgF(x) reveals the basis of substrate binding. | Fletcher, JI., et al. 2002. Structure. 10: 205-13. PMID: 11839306
- The crystal structure of diadenosine tetraphosphate hydrolase from Caenorhabditis elegans in free and binary complex forms. | Bailey, S., et al. 2002. Structure. 10: 589-600. PMID: 11937063
- Diadenosine polyphosphates: postulated mechanisms mediating the cardiac effects. | Stavrou, BM. 2003. Curr Med Chem Cardiovasc Hematol Agents. 1: 151-69. PMID: 15320695
- Influence of diadenosine tetraphosphate (Ap4A) on lipid metabolism. | Rüsing, D. and Verspohl, EJ. 2004. Cell Biochem Funct. 22: 333-8. PMID: 15338474
- Structure and substrate-binding mechanism of human Ap4A hydrolase. | Swarbrick, JD., et al. 2005. J Biol Chem. 280: 8471-81. PMID: 15596429
- 1H, 13C, and 15N resonance assignments of the 17 kDa Ap4A hydrolase from Homo sapiens in the presence and absence of ATP. | Swarbrick, JD., et al. 2005. J Biomol NMR. 31: 181-2. PMID: 15772762
- Effects of dinucleoside polyphosphates on trabecular meshwork cells and aqueous humor outflow facility. | Soto, D., et al. 2005. J Pharmacol Exp Ther. 314: 1042-51. PMID: 15947035
- Prior vasorelaxation enhances diadenosine polyphosphate-induced contractility of rat mesenteric resistance arteries. | Steinmetz, M., et al. 2005. Naunyn Schmiedebergs Arch Pharmacol. 371: 359-63. PMID: 15997394
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AP4A Hydrolase Antibody (F-5) | sc-271410 | 200 µg/ml | $316.00 | |||
AP4A Hydrolase Antibody (F-5): m-IgG Fc BP-HRP Bundle | sc-529177 | 200 µg Ab; 10 µg BP | $354.00 | |||
AP4A Hydrolase Antibody (F-5): m-IgGκ BP-HRP Bundle | sc-521934 | 200 µg Ab, 40 µg BP | $354.00 | |||
AP4A Hydrolase Antibody (F-5): m-IgG1 BP-HRP Bundle | sc-543371 | 200 µg Ab; 20 µg BP | $354.00 | |||
AP4A Hydrolase Antibody (F-5) AC | sc-271410 AC | 500 µg/ml, 25% agarose | $416.00 | |||
AP4A Hydrolase Antibody (F-5) HRP | sc-271410 HRP | 200 µg/ml | $316.00 | |||
AP4A Hydrolase Antibody (F-5) FITC | sc-271410 FITC | 200 µg/ml | $330.00 | |||
AP4A Hydrolase Antibody (F-5) PE | sc-271410 PE | 200 µg/ml | $343.00 | |||
AP4A Hydrolase Antibody (F-5) Alexa Fluor® 488 | sc-271410 AF488 | 200 µg/ml | $357.00 | |||
AP4A Hydrolase Antibody (F-5) Alexa Fluor® 546 | sc-271410 AF546 | 200 µg/ml | $357.00 | |||
AP4A Hydrolase Antibody (F-5) Alexa Fluor® 594 | sc-271410 AF594 | 200 µg/ml | $357.00 | |||
AP4A Hydrolase Antibody (F-5) Alexa Fluor® 647 | sc-271410 AF647 | 200 µg/ml | $357.00 | |||
AP4A Hydrolase Antibody (F-5) Alexa Fluor® 680 | sc-271410 AF680 | 200 µg/ml | $357.00 | |||
AP4A Hydrolase Antibody (F-5) Alexa Fluor® 790 | sc-271410 AF790 | 200 µg/ml | $357.00 |