APIP Antibody (G-2): sc-393194
- APIP Antibody (G-2) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 171-242 mapping at the C-terminus of APIP of human origin
- Anti-APIP Antibody (G-2) is recommended for detection of APIP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-APIP Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of APIP (G-2): sc-393194.
- m-IgG2a BP-HRP is the preferred secondary detection reagent for APIP Antibody (G-2) for WB applications. This reagent is now offered in a bundle with APIP Antibody (G-2) (see ordering information below).
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SEE ALSO...
APIP Antibody (G-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects APIP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). APIP Antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. APIP, or Apaf-1-interacting protein, is a crucial member of the aldolase class II family, characterized by a highly conserved C-terminal region from Caenorhabditis elegans to humans. APIP is ubiquitously expressed, with particularly high levels found in the heart, kidney, and skeletal muscle, highlighting its importance in these vital tissues. Notably, APIP plays a significant role in cellular protection against ischemic damage in muscle tissues by suppressing hypoxia-induced cell death. APIP achieves this by activating key signaling pathways involving Akt and ERK 1/2, which inhibit caspase-9 activation through phosphorylation, while also competing with caspase-9 for binding to the caspase recruitment domain of Apaf-1. By negatively regulating the activation of caspase-9 and Apaf-1-mediated cell death, APIP is essential for maintaining cellular integrity under stress conditions, making APIP a valuable target for research in cell survival mechanisms and potential therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APIP Antibody (G-2) | sc-393194 | 200 µg/ml | $316.00 | |||
APIP Antibody (G-2): m-IgG2a BP-HRP Bundle | sc-545964 | 200 µg Ab; 10 µg BP | $354.00 | |||
APIP Antibody (G-2) AC | sc-393194 AC | 500 µg/ml, 25% agarose | $416.00 | |||
APIP Antibody (G-2) HRP | sc-393194 HRP | 200 µg/ml | $316.00 | |||
APIP Antibody (G-2) FITC | sc-393194 FITC | 200 µg/ml | $330.00 | |||
APIP Antibody (G-2) PE | sc-393194 PE | 200 µg/ml | $343.00 | |||
APIP Antibody (G-2) Alexa Fluor® 488 | sc-393194 AF488 | 200 µg/ml | $357.00 | |||
APIP Antibody (G-2) Alexa Fluor® 546 | sc-393194 AF546 | 200 µg/ml | $357.00 | |||
APIP Antibody (G-2) Alexa Fluor® 594 | sc-393194 AF594 | 200 µg/ml | $357.00 | |||
APIP Antibody (G-2) Alexa Fluor® 647 | sc-393194 AF647 | 200 µg/ml | $357.00 | |||
APIP Antibody (G-2) Alexa Fluor® 680 | sc-393194 AF680 | 200 µg/ml | $357.00 | |||
APIP Antibody (G-2) Alexa Fluor® 790 | sc-393194 AF790 | 200 µg/ml | $357.00 |