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APIP Antibody (G-2): sc-393194

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Datasheets
  • APIP Antibody (G-2) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
  • raised against amino acids 171-242 mapping at the C-terminus of APIP of human origin
  • Anti-APIP Antibody (G-2) is recommended for detection of APIP of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-APIP Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of APIP (G-2): sc-393194.
  • m-IgG2a BP-HRP is the preferred secondary detection reagent for APIP Antibody (G-2) for WB applications. This reagent is now offered in a bundle with APIP Antibody (G-2) (see ordering information below).

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    SEE ALSO...

    APIP Antibody (G-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects APIP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). APIP Antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. APIP, or Apaf-1-interacting protein, is a crucial member of the aldolase class II family, characterized by a highly conserved C-terminal region from Caenorhabditis elegans to humans. APIP is ubiquitously expressed, with particularly high levels found in the heart, kidney, and skeletal muscle, highlighting its importance in these vital tissues. Notably, APIP plays a significant role in cellular protection against ischemic damage in muscle tissues by suppressing hypoxia-induced cell death. APIP achieves this by activating key signaling pathways involving Akt and ERK 1/2, which inhibit caspase-9 activation through phosphorylation, while also competing with caspase-9 for binding to the caspase recruitment domain of Apaf-1. By negatively regulating the activation of caspase-9 and Apaf-1-mediated cell death, APIP is essential for maintaining cellular integrity under stress conditions, making APIP a valuable target for research in cell survival mechanisms and potential therapeutic interventions.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    APIP Antibody (G-2)

    sc-393194
    200 µg/ml
    $316.00

    APIP Antibody (G-2): m-IgG2a BP-HRP Bundle

    sc-545964
    200 µg Ab; 10 µg BP
    $354.00

    APIP Antibody (G-2) AC

    sc-393194 AC
    500 µg/ml, 25% agarose
    $416.00

    APIP Antibody (G-2) HRP

    sc-393194 HRP
    200 µg/ml
    $316.00

    APIP Antibody (G-2) FITC

    sc-393194 FITC
    200 µg/ml
    $330.00

    APIP Antibody (G-2) PE

    sc-393194 PE
    200 µg/ml
    $343.00

    APIP Antibody (G-2) Alexa Fluor® 488

    sc-393194 AF488
    200 µg/ml
    $357.00

    APIP Antibody (G-2) Alexa Fluor® 546

    sc-393194 AF546
    200 µg/ml
    $357.00

    APIP Antibody (G-2) Alexa Fluor® 594

    sc-393194 AF594
    200 µg/ml
    $357.00

    APIP Antibody (G-2) Alexa Fluor® 647

    sc-393194 AF647
    200 µg/ml
    $357.00

    APIP Antibody (G-2) Alexa Fluor® 680

    sc-393194 AF680
    200 µg/ml
    $357.00

    APIP Antibody (G-2) Alexa Fluor® 790

    sc-393194 AF790
    200 µg/ml
    $357.00

    How much sample do you recommend loading on the gel for Western blot detection using APIP (G-2): sc-393194?

    Asked by: Randy McDonald
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
    • y_2025, m_5, d_22, h_5CST
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    Rated 5 out of 5 by from Nice Western Blot data of APIP expressionNice Western Blot data of APIP expression in HeLa whole cell lysate. -SCBT QC
    Date published: 2015-05-03
    Rated 5 out of 5 by from Good Western Blot data of APIP expression in nonGood Western Blot data of APIP expression in non-transfected and human APIP transfected 293 whole cell lysates. -SCBT QC
    Date published: 2014-07-25
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    APIP Antibody (G-2) is rated 5.0 out of 5 by 2.
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