Bad Antibody (C-7): sc-8044

Bad Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bad protein of mouse, rat, and human origin through applications such as western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and enzyme-linked immunosorbent assay. Bad (C-7) antibody is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Bad protein plays a crucial role in the regulation of apoptosis, which is the process of programmed cell death essential for maintaining cellular homeostasis and development. By promoting cell death, Bad acts as a pro-apoptotic member of the Bcl-2 family, counteracting the effects of anti-apoptotic proteins like Bcl-2 and Bcl-xL. The ability of Bad to dimerize with Bcl-xL and Bcl-2, while not interacting with other pro-apoptotic proteins such as Bax, highlights its specific regulatory function in apoptosis. This selective binding is significant because Bad effectively reverses the death-repressing activity of Bcl-xL, thereby facilitating the activation of Bax and promoting apoptosis in response to cellular stress. Understanding Bad function and interactions within the Bcl-2 family is vital for elucidating the mechanisms of cell death and survival, which have implications in cancer research and therapeutic strategies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Bad Antibody (C-7) References:

1. Chkl binds and phosphorylates BAD protein.  |  Han, EK., et al. 2004. Anticancer Res. 24: 3907-10. PMID: 15736430
2. ACTX-8, a cytotoxic L-amino acid oxidase isolated from Agkistrodon acutus snake venom, induces apoptosis in Hela cervical cancer cells.  |  Zhang, L. and Wei, LJ. 2007. Life Sci. 80: 1189-97. PMID: 17275856
3. Docetaxel-induced apoptosis of human melanoma is mediated by activation of c-Jun NH2-terminal kinase and inhibited by the mitogen-activated protein kinase extracellular signal-regulated kinase 1/2 pathway.  |  Mhaidat, NM., et al. 2007. Clin Cancer Res. 13: 1308-14. PMID: 17317842
4. Immunohistochemical analysis of phospho-BAD protein and mutational analysis of BAD gene in gastric carcinomas.  |  Jeong, EG., et al. 2007. APMIS. 115: 976-81. PMID: 17696955
5. BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death.  |  Hockenbery, DM., et al. 1991. Proc Natl Acad Sci U S A. 88: 6961-5. PMID: 1871110
6. Deregulated Bcl-2 gene expression selectively prolongs survival of growth factor-deprived hemopoietic cell lines.  |  Nuñez, G., et al. 1990. J Immunol. 144: 3602-10. PMID: 2184193
7. Comparing the BAD Protein Interactomes in 2D and 3D Cell Culture Using Proximity Labeling.  |  Pirayeshfard, L., et al. 2024. J Proteome Res. 23: 3433-3443. PMID: 38959414
8. Identification of immunosuppressant-induced apoptosis in a murine B-cell line and its prevention by bcl-x but not bcl-2.  |  Gottschalk, AR., et al. 1994. Proc Natl Acad Sci U S A. 91: 7350-4. PMID: 7518929
9. Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak.  |  Kiefer, MC., et al. 1995. Nature. 374: 736-9. PMID: 7715731
10. Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death.  |  Yang, E., et al. 1995. Cell. 80: 285-91. PMID: 7834748
11. BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax.  |  Yin, XM., et al. 1994. Nature. 369: 321-3. PMID: 8183370
12. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death.  |  Oltvai, ZN., et al. 1993. Cell. 74: 609-19. PMID: 8358790

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bad Antibody (C-7) has 262 citations in a variety of scientific publications.

Primary antibodies like Anti-Bad Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bad Antibody (C-7) Specifications

Alternate Product Name: Bad

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-168

Epitope Target: Bad

Epitope Species: human

Product Reactivity/Detection: Bad

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 262 citations in various scientific publications

Available Conjugates/Forms for Bad Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-Bad antibody is p-Bad Antibody (C-10): sc-166932.