C5 Antibody (561): sc-52636

C5 Antibody (561) is a mouse monoclonal IgG2a antibody that detects C5 protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-C5 antibody (561) is available as a non-conjugated format and plays a crucial role in the complement system, which is essential for immune defense. C5 (561) antibody recognizes a key component of the complement cascade, where activation leads to the generation of two biologically active fragments: C5a, a potent anaphylatoxin, and C5b, which initiates the formation of the membrane attack complex. The C5a fragment binds to specific G protein-coupled receptors on myeloid leukocytes, triggering a series of pro-inflammatory responses that include cellular degranulation, smooth muscle contraction, and cytokine release. These processes orchestrate an effective immune response against pathogens. The C5 precursor undergoes significant post-translational modifications, including cleavage by C5 convertase, which is critical for activation and function. Proper regulation of C5 and its fragments is essential, as dysregulation can lead to pathological conditions such as asthma and bacterial meningitis, highlighting C5 (561) antibody′s importance in research and therapeutic applications.

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C5 Antibody (561) References:

1. Modulation of C3a activity: internalization of the human C3a receptor and its inhibition by C5a.  |  Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
2. Ligand-induced phosphorylation of anaphylatoxin receptors C3aR and C5aR is mediated by 'G protein-coupled receptor kinases.  |  Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
3. A role for the C3a anaphylatoxin receptor in the effector phase of asthma.  |  Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
4. C5 neoepitopes appearing during activation.  |  Inoue, K. 1989. Complement Inflamm. 6: 219-22. PMID: 2472923
5. Human complement component C3: cDNA coding sequence and derived primary structure.  |  de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
6. Complement C5 Gene Confers Risk for Acute Anterior Uveitis.  |  Xu, D., et al. 2015. Invest Ophthalmol Vis Sci. 56: 4954-60. PMID: 26230759
7. Complement C5 Protein as a Marker of Subclinical Atherosclerosis.  |  Martínez-López, D., et al. 2020. J Am Coll Cardiol. 75: 1926-1941. PMID: 32327104
8. The allosteric modulation of complement C5 by knob domain peptides.  |  Macpherson, A., et al. 2021. Elife. 10: PMID: 33570492
9. The conversion of human complement component C5 into fragment C5b by the alternative-pathway C5 convertase.  |  DiScipio, RG. 1981. Biochem J. 199: 497-504. PMID: 6918218
10. Mitogen-activated protein kinase activation requires two signal inputs from the human anaphylatoxin C5a receptor.  |  Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
11. Phylogeny of the C3/C4/C5 complement-component gene family indicates that C5 diverged first.  |  Hughes, AL. 1994. Mol Biol Evol. 11: 417-25. PMID: 8015436
12. TNF-alpha-mediated expression of the receptor for anaphylatoxin C5a on neurons in experimental Listeria meningoencephalitis.  |  Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-C5 Antibody (561) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-C5 Antibody (561) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

C5 Antibody (561) Specifications

Alternate Product Name: Complement Component 5

Product Clone Name: 561

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: C5

Epitope Species: human

Product Reactivity/Detection: the N-terminus of C5, intact C5 and C5a

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for C5 Antibody (561): N/A

The preferred C5b-9 antibody is C5b-9 Antibody (aE11): sc-58935.