CD154 Antibody (F-1): sc-374635

CD154 Antibody (F-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects CD154 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD154 (F-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD154, also known as CD40 ligand, plays a crucial role in the immune response by mediating interactions between T cells and B cells, which are essential for B cell activation and differentiation into antibody-producing cells. CD154 is expressed on activated T cells and binds to CD40 on B cells, initiating a signaling cascade that promotes B cell proliferation and immunoglobulin class switching, particularly to IgE in the presence of cytokines such as interleukin-4 and interleukin-13. This interaction is vital for the adaptive immune response, and dysregulation of CD154 expression can lead to various immunological disorders, including hyper-IgM immunodeficiency syndrome type 1, where mutations in CD154 gene result in impaired T-B cell interactions. Furthermore, abnormal levels of CD154 are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events, highlighting CD154′s importance not only in immune regulation but also in cardiovascular health.

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CD154 Antibody (F-1) References:

1. CD154 and type-1 cytokine response: from hyper IgM syndrome to human immunodeficiency virus infection.  |  Subauste, CS. 2002. J Infect Dis. 185 Suppl 1: S83-9. PMID: 11865444
2. Enhanced NFATc1 nuclear occupancy causes T cell activation independent of CD28 costimulation.  |  Pan, M., et al. 2007. J Immunol. 178: 4315-21. PMID: 17371988
3. Resistance to the induction of mixed chimerism in spontaneously diabetic NOD mice depends on the CD40/CD154 pathway and donor MHC disparity.  |  Luo, B., et al. 2007. Ann N Y Acad Sci. 1103: 94-102. PMID: 17376827
4. CD154 (CD40L): A novel aid to document nonimmediate hypersensitivity to amoxicillin or amoxicillin clavulanic acid.  |  Van Gasse, AL., et al. 2020. Clin Exp Allergy. 50: 640-642. PMID: 32056306
5. CD154 Induces Interleukin-6 Secretion by Kidney Tubular Epithelial Cells under Hypoxic Conditions: Inhibition by Chloroquine.  |  Dewitte, A., et al. 2020. Mediators Inflamm. 2020: 6357046. PMID: 32089648
6. A novel RING finger protein interacts with the cytoplasmic domain of CD40.  |  Hu, HM., et al. 1994. J Biol Chem. 269: 30069-72. PMID: 7527023
7. Involvement of CRAF1, a relative of TRAF, in CD40 signaling.  |  Cheng, G., et al. 1995. Science. 267: 1494-8. PMID: 7533327
8. B-cell activation by helper T-cell membranes.  |  Kehry, MR. and Hodgkin, PD. 1994. Crit Rev Immunol. 14: 221-38. PMID: 7538767
9. CD40 and its ligand: central players in B lymphocyte survival, growth, and differentiation.  |  Gordon, J. 1995. Blood Rev. 9: 53-6. PMID: 7540904
10. Expression of the CD40 ligand in T lymphocytes and induction of IgE isotype switching.  |  Fuleihan, R., et al. 1995. Int Arch Allergy Immunol. 107: 43-4. PMID: 7542098
11. TRAF2-mediated activation of NF-kappa B by TNF receptor 2 and CD40.  |  Rothe, M., et al. 1995. Science. 269: 1424-7. PMID: 7544915
12. A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor.  |  Rothe, M., et al. 1994. Cell. 78: 681-92. PMID: 8069916

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD154 Antibody (F-1) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-CD154 Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD154 Antibody (F-1) Specifications

Alternate Product Name: CD40L

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 231-267

Epitope Target: CD154

Epitope Species: human

Product Reactivity/Detection: CD154

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for CD154 Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin