CD28 Antibody (152-2E10): sc-21770
- CD28 Antibody (152-2E10) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against PHA stimulated human PBL
- recommended for detection of CD28 of human origin by WB, IP and FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD28 Antibody (152-2E10) for WB applications. These reagents are now offered in bundles with CD28 Antibody (152-2E10) (see ordering information below).
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CD28 Antibody (152-2E10) is a mouse monoclonal IgG1 antibody that detects CD28 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). CD28 plays a crucial role in T cell activation and survival by providing essential co-stimulatory signals upon binding to CD80 and CD86 ligands, which are expressed on antigen-presenting cells. This interaction is vital for the full activation of T cells, as CD28 enhances proliferation and cytokine production, ultimately influencing the immune response. CD28 is predominantly expressed on resting T cells, while CTLA-4 serves as a negative regulator and is upregulated on activated T cells, highlighting the delicate balance of immune activation and inhibition. Anti-CD28 antibody (152-2E10) is particularly valuable for researchers studying T cell biology and the mechanisms of immune regulation, enabling detailed analysis of CD28 expression and function in various experimental settings.
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CD28 Antibody (152-2E10) References:
- CD28 superagonists: mode of action and therapeutic potential. | Hünig, T. and Dennehy, K. 2005. Immunol Lett. 100: 21-8. PMID: 16054703
- CD28 co-stimulation down regulates Th17 development. | Bouguermouh, S., et al. 2009. PLoS One. 4: e5087. PMID: 19333372
- The clinical utility of inhibiting CD28-mediated costimulation. | Linsley, PS. and Nadler, SG. 2009. Immunol Rev. 229: 307-21. PMID: 19426230
- CD28 deletion improves obesity-induced liver steatosis but increases adiposity in mice. | Poggi, M., et al. 2015. Int J Obes (Lond). 39: 977-85. PMID: 25771927
- Viral-induced CD28 loss evokes costimulation independent alloimmunity. | Mou, D., et al. 2015. J Surg Res. 196: 241-6. PMID: 25801976
- Membrane Rafts in T Cell Activation: A Spotlight on CD28 Costimulation. | Zumerle, S., et al. 2017. Front Immunol. 8: 1467. PMID: 29163534
- Tasmanian devil CD28 and CTLA4 capture CD80 and CD86 from adjacent cells. | Wong, C., et al. 2021. Dev Comp Immunol. 115: 103882. PMID: 33039410
- Sialic Acid Ligands of CD28 Suppress Costimulation of T Cells. | Edgar, LJ., et al. 2021. ACS Cent Sci. 7: 1508-1515. PMID: 34584952
- Increased Percentage of CD8+CD28- Regulatory T Cells With Fingolimod Therapy in Multiple Sclerosis. | Houston, TW., et al. 2023. Neurol Neuroimmunol Neuroinflamm. 10: PMID: 36535763
- Regulation of T cell activation by CD28 and CTLA4. | Noel, PJ., et al. 1996. Adv Exp Med Biol. 406: 209-17. PMID: 8910687
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD28 Antibody (152-2E10) | sc-21770 | 200 µg/ml | $316.00 | |||
CD28 Antibody (152-2E10): m-IgG Fc BP-HRP Bundle | sc-536666 | 200 µg Ab; 10 µg BP | $354.00 | |||
CD28 Antibody (152-2E10): m-IgGκ BP-HRP Bundle | sc-533825 | 200 µg Ab; 40 µg BP | $354.00 | |||
CD28 Antibody (152-2E10) FITC | sc-21770 FITC | 200 µg/ml | $330.00 | |||
CD28 Antibody (152-2E10) PE | sc-21770 PE | 200 µg/ml | $343.00 |