CD32-B Antibody (F-4): sc-365864

CD32-B Antibody (F-4) is a mouse monoclonal IgG1 antibody that detects CD32-B of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CD32-B (F-4) antibody is available in a non-conjugated form, providing flexibility for various experimental needs. CD32, also known as Fc gamma RII, serves as a low-affinity receptor for the Fc fragment of aggregated immunoglobulin G (IgG), playing a crucial role in the immune response by mediating the clearance of immune complexes through macrophages. This function is vital for maintaining homeostasis and preventing autoimmune reactions. Additionally, CD32-B is involved in the regulation of antibody production by B cells, where CD32-B interaction with IgG delivers a negative signal that modulates B cell activity. CD32-B exists in several isoforms, produced by alternative splicing of three distinct genes (A, B, and C), which are designated FcgRIIA, FcgRIIB1, FcgRIIB3, and FcgRIIC. These isoforms are expressed on various immune cells, including monocytes, placental trophoblasts, and endothelial cells, with specific forms present on B lymphocytes and neutrophils, highlighting CD32-B′s diverse roles in immune regulation and response.

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CD32-B Antibody (F-4) References:

1. Characterization and crystallization of soluble human Fc gamma receptor II (CD32) isoforms produced in insect cells.  |  Sondermann, P., et al. 1999. Biochemistry. 38: 8469-77. PMID: 10387093
2. Fc receptors.  |  Ravetch, JV. and Kinet, JP. 1991. Annu Rev Immunol. 9: 457-92. PMID: 1910686
3. CD19 and CD32b differentially regulate human B cell responsiveness.  |  Karnell, JL., et al. 2014. J Immunol. 192: 1480-90. PMID: 24442430
4. Human IgG Fc receptor (hFcRII; CD32) exists as multiple isoforms in macrophages, lymphocytes and IgG-transporting placental epithelium.  |  Stuart, SG., et al. 1989. EMBO J. 8: 3657-66. PMID: 2531080
5. CD32b expression is down-regulated on double-negative memory B cells in patients with Hashimoto's thyroiditis.  |  Liu, Y., et al. 2017. Mol Cell Endocrinol. 440: 1-7. PMID: 27832986
6. Binding characteristics of dimeric IgG subclass complexes to human neutrophils.  |  Huizinga, TW., et al. 1989. J Immunol. 142: 2359-64. PMID: 2784461
7. Development of Anti-CD32b Antibodies with Enhanced Fc Function for the Treatment of B and Plasma Cell Malignancies.  |  Lu, H., et al. 2020. Mol Cancer Ther. 19: 2089-2104. PMID: 32847974
8. DPP4/CD32b/NF-κB Circuit: A Novel Druggable Target for Inhibiting CRP-Driven Diabetic Nephropathy.  |  Tang, PM., et al. 2021. Mol Ther. 29: 365-375. PMID: 32956626
9. Homogeneously high expression of CD32b makes it a potential target for CAR-T therapy for chronic lymphocytic leukemia.  |  Wang, G., et al. 2021. J Hematol Oncol. 14: 149. PMID: 34530888
10. The CD16 and CD32b Fc-gamma receptors regulate antibody-mediated responses in mouse natural killer cells.  |  Aguilar, OA., et al. 2023. J Leukoc Biol. 113: 27-40. PMID: 36822164
11. Crosslinking of surface immunoglobulin and Fc receptors on B lymphocytes inhibits stimulation of inositol phospholipid breakdown via the antigen receptors.  |  Bijsterbosch, MK. and Klaus, GG. 1985. J Exp Med. 162: 1825-36. PMID: 3877778
12. The Immunosuppressive Receptor CD32b Regulation of Macrophage Polarization and Its Implications in Tumor Progression.  |  Chuang, HJ., et al. 2024. Int J Mol Sci. 25: PMID: 39273683

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD32-B Antibody (F-4) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-CD32-B Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD32-B Antibody (F-4) Specifications

Alternate Product Name: CD32-B

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 285-310

Epitope Target: CD32-B

Epitope Species: human

Product Reactivity/Detection: CD32-B

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for CD32-B Antibody (F-4): P: Blocking Peptide