CDD Antibody (D-5): sc-365292

CDD Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects CDD of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CDD (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytidine deaminase (CDD or CDA) plays a crucial role in the metabolism of nucleoside analogs, specifically catalyzing the deamination of chemotherapeutic agents like Ara-C and 5-azacytidine, which are vital in treating acute myeloid leukemia (AML). The effectiveness of Ara-C relies on its phosphorylation by deoxycytidine kinase (DCK), and increased activity of CDD can lead to resistance against this treatment, making CDD a significant factor in the unsuccessful management of AML. Additionally, CDD is involved in the scavenging of cytidine and 2′-deoxycytidine, contributing to UMP synthesis, which is essential for nucleotide metabolism. CDD can form homotetramers and is predominantly expressed in granulocytes, highlighting CDD′s importance in both hematopoiesis and the immune response.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CDD Antibody (D-5) References:

1. Disparate roles of ATR and ATM in immunoglobulin class switch recombination and somatic hypermutation.  |  Pan-Hammarström, Q., et al. 2006. J Exp Med. 203: 99-110. PMID: 16390936
2. Zebularine: a unique molecule for an epigenetically based strategy in cancer chemotherapy.  |  Marquez, VE., et al. 2005. Ann N Y Acad Sci. 1058: 246-54. PMID: 16394141
3. The nucleotide targets of somatic mutation and the role of selection in immunoglobulin heavy chains of a teleost fish.  |  Yang, F., et al. 2006. J Immunol. 176: 1655-67. PMID: 16424195
4. H2AX prevents DNA breaks from progressing to chromosome breaks and translocations.  |  Franco, S., et al. 2006. Mol Cell. 21: 201-14. PMID: 16427010
5. Identification of functional single nucleotide polymorphism haplotypes in the cytidine deaminase promoter.  |  Fitzgerald, SM., et al. 2006. Hum Genet. 119: 276-83. PMID: 16446974
6. Cytidine deaminase: a new genetic polymorphism demonstrated in human granulocytes.  |  Teng, YS., et al. 1975. Am J Hum Genet. 27: 492-7. PMID: 50738
7. Human cytidine deaminase: purification of enzyme, cloning, and expression of its complementary DNA.  |  Laliberté, J. and Momparler, RL. 1994. Cancer Res. 54: 5401-7. PMID: 7923172
8. Cloning of a functional cDNA for human cytidine deaminase (CDD) and its use as a marker of monocyte/macrophage differentiation.  |  Kühn, K., et al. 1993. Biochem Biophys Res Commun. 190: 1-7. PMID: 8422236
9. Activity of thymidine kinase and of polymerase alpha as well as activity and gene expression of deoxycytidine deaminase in leukemic blasts are correlated with clinical response in the setting of granulocyte-macrophage colony-stimulating factor-based priming before and during TAD-9 induction therapy in acute myeloid leukemia.  |  Jahns-Streubel, G., et al. 1997. Blood. 90: 1968-76. PMID: 9292531
10. Isolation and characterization of the gene coding for human cytidine deaminase.  |  Demontis, S., et al. 1998. Biochim Biophys Acta. 1443: 323-33. PMID: 9878810

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CDD Antibody (D-5) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-CDD Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CDD Antibody (D-5) Specifications

Alternate Product Name: CDD

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-146

Epitope Target: CDD

Epitope Species: human

Product Reactivity/Detection: CDD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for CDD Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin