C5β Antibody (E-8): sc-398247

C5β Antibody (E-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects C5β protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). C5β (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. C5β protein plays a crucial role in the complement system, which is essential for immune defense against pathogens. Specifically, C5β is generated from the cleavage of the C5 precursor, which is first processed to remove four basic residues, resulting in two chains, α and β, linked by a disulfide bond. The activation of C5 by C5 convertase leads to the release of C5α anaphylatoxin, a potent mediator of inflammation, and the generation of C5β, which is involved in the formation of the membrane attack complex. This complex is vital for lysing pathogens and facilitating the clearance of immune complexes. The interaction of C5β with other complement components and receptors on myeloid leukocytes triggers proinflammatory responses, including cellular degranulation and cytokine release, which are critical for mounting an effective immune response. Understanding the function and dynamics of C5β is important for elucidating its role in various pathophysiological conditions, including asthma and bacterial infections, where complement activation is often dysregulated.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

C5β Antibody (E-8) References:

1. Modulation of C3a activity: internalization of the human C3a receptor and its inhibition by C5a.  |  Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
2. Ligand-induced phosphorylation of anaphylatoxin receptors C3aR and C5aR is mediated by 'G protein-coupled receptor kinases.  |  Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
3. A role for the C3a anaphylatoxin receptor in the effector phase of asthma.  |  Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
4. Human complement component C3: cDNA coding sequence and derived primary structure.  |  de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
5. Mitogen-activated protein kinase activation requires two signal inputs from the human anaphylatoxin C5a receptor.  |  Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
6. TNF-alpha-mediated expression of the receptor for anaphylatoxin C5a on neurons in experimental Listeria meningoencephalitis.  |  Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-C5β Antibody (E-8) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-C5β Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

C5β Antibody (E-8) Specifications

Alternate Product Name: C5β

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 562-589

Epitope Target: C5

Epitope Species: human

Product Reactivity/Detection: C5 precursor and β chain

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for C5β Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, P: Blocking Peptide, PE: Phycoerythrin