GITR Antibody (621): sc-53972

GITR Antibody (621) is a mouse monoclonal IgG1 kappa light chain antibody that detects GITR protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). GITR (621) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The glucocorticoid-induced tumor necrosis factor receptor family-related protein (GITR) plays a crucial role in modulating immune responses, particularly in T lymphocytes, where GITR is preferentially expressed in the thymus, spleen, and lymph nodes. GITR is a member of the tumor necrosis factor receptor (TNFR) superfamily, which is characterized by type I transmembrane glycoproteins that are integral to various cellular functions, including proliferation, differentiation, and programmed cell death. The structure of GITR includes a cysteine-rich extracellular domain that facilitates ligand binding and an intracellular death domain that is essential for signal transduction. This structural configuration allows GITR to interact with other proteins in the TNFR superfamily, such as Ox40, 4-1BB, and CD27, thereby influencing the activation of critical signaling pathways like NFκB, which promotes cell survival, and caspase pathways, which lead to apoptosis. Understanding the structure and function of GITR is vital for developing therapeutic strategies aimed at enhancing or inhibiting immune responses in various diseases, including cancer and autoimmune disorders.

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GITR Antibody (621) References:

1. Identification of a new member of the tumor necrosis factor family and its receptor, a human ortholog of mouse GITR.  |  Gurney, AL., et al. 1999. Curr Biol. 9: 215-8. PMID: 10074428
2. Glucocorticoid hormone-induced modulation of gene expression and regulation of T-cell death: role of GITR and GILZ, two dexamethasone-induced genes.  |  Riccardi, C., et al. 1999. Cell Death Differ. 6: 1182-9. PMID: 10637434
3. Gene structure and chromosomal assignment of mouse GITR, a member of the tumor necrosis factor/nerve growth factor receptor family.  |  Nocentini, G., et al. 2000. DNA Cell Biol. 19: 205-17. PMID: 10798444
4. GITR+ regulatory T cells in the treatment of autoimmune diseases.  |  Petrillo, MG., et al. 2015. Autoimmun Rev. 14: 117-26. PMID: 25449679
5. The Role of GITR/GITRL Interaction in Autoimmune Diseases.  |  Tian, J., et al. 2020. Front Immunol. 11: 588682. PMID: 33163004
6. GITR and TIGIT immunotherapy provokes divergent multicellular responses in the tumor microenvironment of gastrointestinal cancers.  |  Sathe, A., et al. 2023. Genome Med. 15: 100. PMID: 38008725
7. GITR exacerbates lysophosphatidylcholine-induced macrophage pyroptosis in sepsis via posttranslational regulation of NLRP3.  |  Liang, S., et al. 2024. Cell Mol Immunol. 21: 674-688. PMID: 38740925
8. Exploring the Role of GITR/GITRL Signaling: From Liver Disease to Hepatocellular Carcinoma.  |  Papadakos, SP., et al. 2024. Cancers (Basel). 16: PMID: 39061246
9. Structural and biological features of the TNF receptor and TNF ligand superfamilies: interactive signals in the pathobiology of Hodgkin's disease.  |  Gruss, HJ., et al. 1996. Ann Oncol. 7 Suppl 4: 19-26. PMID: 8836404
10. Molecular, structural, and biological characteristics of the tumor necrosis factor ligand superfamily.  |  Gruss, HJ. 1996. Int J Clin Lab Res. 26: 143-59. PMID: 8905447
11. A new member of the tumor necrosis factor/nerve growth factor receptor family inhibits T cell receptor-induced apoptosis.  |  Nocentini, G., et al. 1997. Proc Natl Acad Sci U S A. 94: 6216-21. PMID: 9177197
12. Modulation of life and death by the TNF receptor superfamily.  |  Baker, SJ. and Reddy, EP. 1998. Oncogene. 17: 3261-70. PMID: 9916988

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GITR Antibody (621) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-GITR Antibody (621) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GITR Antibody (621) Specifications

Alternate Product Name: GITR

Product Clone Name: 621

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: GITR

Epitope Species: human

Product Reactivity/Detection: GITR

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for GITR Antibody (621): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin