RAG-1 Antibody (D-5): sc-377127

RAG-1 Antibody (D-5) is a mouse monoclonal IgM kappa antibody that detects RAG-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-RAG-1 antibody (D-5) is available as the non-conjugated form, which plays a crucial role in the immune system by facilitating V(D)J recombination, a mechanism essential for generating diverse antigen receptors in B and T lymphocytes. This process occurs in lymphoid organs, where RAG-1, along with RAG-2, orchestrates the rearrangement of immunoglobulin and T cell receptor genes from multiple germline segments, allowing for the production of a vast array of antibodies and T cell receptors vital for adaptive immunity. RAG-1′s importance extends beyond immune diversity to potential implications in immunodeficiencies and lymphoid malignancies when mutations occur. Using RAG-1 (D-5) antibody in various applications helps researchers gain insights into immune development mechanisms and underlying causes of related diseases, making RAG-1 (D-5) monoclonal antibody an invaluable tool in immunological research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RAG-1 Antibody (D-5) References:

1. A RAG-1/RAG-2 tetramer supports 12/23-regulated synapsis, cleavage, and transposition of V(D)J recombination signals.  |  Swanson, PC. 2002. Mol Cell Biol. 22: 7790-801. PMID: 12391148
2. RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.  |  Shinkai, Y., et al. 1992. Cell. 68: 855-67. PMID: 1547487
3. RAG-1-deficient mice have no mature B and T lymphocytes.  |  Mombaerts, P., et al. 1992. Cell. 68: 869-77. PMID: 1547488
4. V(D)J recombination: molecular biology and regulation.  |  Schatz, DG., et al. 1992. Annu Rev Immunol. 10: 359-83. PMID: 1590991
5. RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination.  |  Oettinger, MA., et al. 1990. Science. 248: 1517-23. PMID: 2360047
6. The V(D)J recombination activating gene, RAG-1.  |  Schatz, DG., et al. 1989. Cell. 59: 1035-48. PMID: 2598259
7. Lessons Learned From RAG-1-Deficient Mice in Hypertension.  |  Rios, FJ., et al. 2020. Hypertension. 75: 935-937. PMID: 32078397
8. Potential application of bioemulsifier RAG-1 as an anti-staling agent in flat bread quality.  |  Sadeghi, H., et al. 2023. J Food Sci Technol. 60: 2619-2627. PMID: 37599842
9. Expression of human recombination activating genes (RAG-1 and RAG-2) in lymphoma.  |  Knecht, H., et al. 1994. Leuk Lymphoma. 15: 399-403. PMID: 7873997
10. RAG-1 and RAG-2 are not sufficient to direct all phases of immunoglobulin gene rearrangement in pre-B-cell lines.  |  Wang, LC. and Rosenberg, N. 1993. Mol Cell Biol. 13: 3890-9. PMID: 8321196
11. Regulation of V(D)J recombination activator protein RAG-2 by phosphorylation.  |  Lin, WC. and Desiderio, S. 1993. Science. 260: 953-9. PMID: 8493533
12. RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development.  |  Chen, J., et al. 1993. Proc Natl Acad Sci U S A. 90: 4528-32. PMID: 8506294

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RAG-1 Antibody (D-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-RAG-1 Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RAG-1 Antibody (D-5) Specifications

Alternate Product Name: RAG-1

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 727-765

Epitope Target: RAG-1

Epitope Species: human

Product Reactivity/Detection: RAG-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for RAG-1 Antibody (D-5): P: Blocking Peptide