Siglec-5 Antibody (H-7): sc-377005
- Siglec-5 Antibody (H-7) is a mouse monoclonal IgG2a κ Siglec-5 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 457-520 mapping near the C-terminus of Siglec-5 of human origin
- Siglec-5 Antibody (H-7) is recommended for detection of Siglec-5 of human origin by WB, IP, IF and ELISA
- Anti-Siglec-5 Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Siglec-5 (H-7): sc-377005.
- m-IgG2a BP-HRP is the preferred secondary detection reagent for Siglec-5 Antibody (H-7) for WB applications. This reagent is now offered in a bundle with Siglec-5 Antibody (H-7) (see ordering information below).
Siglec-5 (H-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects human Siglec-5 protein by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Siglec-5 (H-7) antibody is available unconjugated and in multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and various Alexa Fluor® conjugates. Two families of mammalian lectin-like adhesion molecules bind glycoconjugate ligands in a sialic acid-dependent manner: selectins and sialoadhesins. Sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members recognizing sialylated ligands. Siglec-5 binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. Four isoforms of hSiglec-5 exist with three (hSiglec-5-3L and -3C) or four (hSiglec-5-4L and -4S) extracellular domains linked to long (hSiglec-5-3L and -4L) or short (hSiglec-5-4S) cytoplasmic tails or as a soluble isoform (hSiglec-5-3C). Siglec-5 is expressed by monocytes and neutrophils, but is absent from leukemic cell lines representing early stages of myelomonocytic differentiation. Siglec-5 may play a role in diagnosing and monitoring acute myeloid leukemia (AML).
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Siglec-5 Antibody (H-7) References:
- Human Siglec-5: tissue distribution, novel isoforms and domain specificities for sialic acid-dependent ligand interactions. | Connolly, NP., et al. 2002. Br J Haematol. 119: 221-38. PMID: 12358929
- Characterization of Siglec-5 (CD170) expression and functional activity of anti-Siglec-5 antibodies on human phagocytes. | Erickson-Miller, CL., et al. 2003. Exp Hematol. 31: 382-8. PMID: 12763136
- Identification of the CD33-related Siglec receptor, Siglec-5 (CD170), as a useful marker in both normal myelopoiesis and acute myeloid leukaemias. | Virgo, P., et al. 2003. Br J Haematol. 123: 420-30. PMID: 14617000
- Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation. | Avril, T., et al. 2005. J Biol Chem. 280: 19843-51. PMID: 15769739
- Sialoside-binding macrophage lectins in phagocytosis of apoptotic bodies. | Rapoport, EM., et al. 2005. Biochemistry (Mosc). 70: 330-8. PMID: 15823088
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Siglec-5 Antibody (H-7) | sc-377005 | 200 µg/ml | $316.00 | |||
Siglec-5 Antibody (H-7): m-IgG2a BP-HRP Bundle | sc-545951 | 200 µg Ab; 10 µg BP | $354.00 | |||
Siglec-5 Antibody (H-7) AC | sc-377005 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Siglec-5 Antibody (H-7) HRP | sc-377005 HRP | 200 µg/ml | $316.00 | |||
Siglec-5 Antibody (H-7) FITC | sc-377005 FITC | 200 µg/ml | $330.00 | |||
Siglec-5 Antibody (H-7) PE | sc-377005 PE | 200 µg/ml | $343.00 | |||
Siglec-5 Antibody (H-7) Alexa Fluor® 488 | sc-377005 AF488 | 200 µg/ml | $357.00 | |||
Siglec-5 Antibody (H-7) Alexa Fluor® 546 | sc-377005 AF546 | 200 µg/ml | $357.00 | |||
Siglec-5 Antibody (H-7) Alexa Fluor® 594 | sc-377005 AF594 | 200 µg/ml | $357.00 | |||
Siglec-5 Antibody (H-7) Alexa Fluor® 647 | sc-377005 AF647 | 200 µg/ml | $357.00 | |||
Siglec-5 Antibody (H-7) Alexa Fluor® 680 | sc-377005 AF680 | 200 µg/ml | $357.00 | |||
Siglec-5 Antibody (H-7) Alexa Fluor® 790 | sc-377005 AF790 | 200 µg/ml | $357.00 |