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Siglec-5 Antibody (H-7): sc-377005

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Datasheets
  • Siglec-5 Antibody (H-7) is a mouse monoclonal IgG2a κ Siglec-5 antibody, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 457-520 mapping near the C-terminus of Siglec-5 of human origin
  • Siglec-5 Antibody (H-7) is recommended for detection of Siglec-5 of human origin by WB, IP, IF and ELISA
  • Anti-Siglec-5 Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Siglec-5 (H-7): sc-377005.
  • m-IgG2a BP-HRP is the preferred secondary detection reagent for Siglec-5 Antibody (H-7) for WB applications. This reagent is now offered in a bundle with Siglec-5 Antibody (H-7) (see ordering information below).

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SEE ALSO...

Siglec-5 (H-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects human Siglec-5 protein by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Siglec-5 (H-7) antibody is available unconjugated and in multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and various Alexa Fluor® conjugates. Two families of mammalian lectin-like adhesion molecules bind glycoconjugate ligands in a sialic acid-dependent manner: selectins and sialoadhesins. Sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members recognizing sialylated ligands. Siglec-5 binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. Four isoforms of hSiglec-5 exist with three (hSiglec-5-3L and -3C) or four (hSiglec-5-4L and -4S) extracellular domains linked to long (hSiglec-5-3L and -4L) or short (hSiglec-5-4S) cytoplasmic tails or as a soluble isoform (hSiglec-5-3C). Siglec-5 is expressed by monocytes and neutrophils, but is absent from leukemic cell lines representing early stages of myelomonocytic differentiation. Siglec-5 may play a role in diagnosing and monitoring acute myeloid leukemia (AML).

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Siglec-5 Antibody (H-7) References:

  1. Human Siglec-5: tissue distribution, novel isoforms and domain specificities for sialic acid-dependent ligand interactions.  |  Connolly, NP., et al. 2002. Br J Haematol. 119: 221-38. PMID: 12358929
  2. Characterization of Siglec-5 (CD170) expression and functional activity of anti-Siglec-5 antibodies on human phagocytes.  |  Erickson-Miller, CL., et al. 2003. Exp Hematol. 31: 382-8. PMID: 12763136
  3. Identification of the CD33-related Siglec receptor, Siglec-5 (CD170), as a useful marker in both normal myelopoiesis and acute myeloid leukaemias.  |  Virgo, P., et al. 2003. Br J Haematol. 123: 420-30. PMID: 14617000
  4. Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation.  |  Avril, T., et al. 2005. J Biol Chem. 280: 19843-51. PMID: 15769739
  5. Sialoside-binding macrophage lectins in phagocytosis of apoptotic bodies.  |  Rapoport, EM., et al. 2005. Biochemistry (Mosc). 70: 330-8. PMID: 15823088

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Siglec-5 Antibody (H-7)

sc-377005
200 µg/ml
$316.00

Siglec-5 Antibody (H-7): m-IgG2a BP-HRP Bundle

sc-545951
200 µg Ab; 10 µg BP
$354.00

Siglec-5 Antibody (H-7) AC

sc-377005 AC
500 µg/ml, 25% agarose
$416.00

Siglec-5 Antibody (H-7) HRP

sc-377005 HRP
200 µg/ml
$316.00

Siglec-5 Antibody (H-7) FITC

sc-377005 FITC
200 µg/ml
$330.00

Siglec-5 Antibody (H-7) PE

sc-377005 PE
200 µg/ml
$343.00

Siglec-5 Antibody (H-7) Alexa Fluor® 488

sc-377005 AF488
200 µg/ml
$357.00

Siglec-5 Antibody (H-7) Alexa Fluor® 546

sc-377005 AF546
200 µg/ml
$357.00

Siglec-5 Antibody (H-7) Alexa Fluor® 594

sc-377005 AF594
200 µg/ml
$357.00

Siglec-5 Antibody (H-7) Alexa Fluor® 647

sc-377005 AF647
200 µg/ml
$357.00

Siglec-5 Antibody (H-7) Alexa Fluor® 680

sc-377005 AF680
200 µg/ml
$357.00

Siglec-5 Antibody (H-7) Alexa Fluor® 790

sc-377005 AF790
200 µg/ml
$357.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with Siglec-5 (H-7): sc-377005 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_5, d_22, h_5CST
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Rated 5 out of 5 by from Great Western blot data of SiglecGreat Western blot data of Siglec-5 expression in non-transfected and human Siglec-5 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-09-18
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Siglec-5 Antibody (H-7) is rated 5.0 out of 5 by 1.
  • y_2025, m_5, d_22, h_5
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