caspase-8 Antibody (8CSP03): sc-56070

caspase-8 Antibody (8CSP03) is a mouse monoclonal IgG1 kappa light chain antibody that detects the caspase-8 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-caspase-8 antibody (8CSP03) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Caspase-8 plays a crucial role in the apoptotic signaling pathway, acting as an initiator caspase that activates downstream effector caspases through the cleavage of their inactive precursors. This process is vital for the regulation of programmed cell death, particularly in response to death receptor signaling via tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and Fas (TNFRSF6). Caspase-8 is primarily located in the cytoplasm and is recruited to the death-inducing signaling complex (DISC) upon binding to the adaptor molecule FADD, where activation occurs. The active form of caspase-8, a heterodimer composed of p18 and p10 subunits, is then released from the DISC to propagate the apoptotic signal. Caspase-8 is widely expressed, with the highest levels found in peripheral blood leukocytes, thymus, liver, and spleen. Mutations or defects in the CASP8 gene can lead to caspase-8 deficiency disorder (CASP8D), which is associated with splenomegaly and impaired apoptosis of peripheral blood leukocytes, potentially resulting in immunodeficiency due to compromised activation of T lymphocytes, natural killer cells, and B lymphocytes.

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caspase-8 Antibody (8CSP03) References:

1. Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction.  |  Wesselborg, S., et al. 1999. Blood. 93: 3053-63. PMID: 10216102
2. Involvement of FADD and caspase-8 signalling in detachment-induced apoptosis.  |  Rytömaa, M., et al. 1999. Curr Biol. 9: 1043-6. PMID: 10508619
3. The atomic-resolution structure of human caspase-8, a key activator of apoptosis.  |  Watt, W., et al. 1999. Structure. 7: 1135-43. PMID: 10508785
4. Pro-caspase-8 is predominantly localized in mitochondria and released into cytoplasm upon apoptotic stimulation.  |  Qin, ZH., et al. 2001. J Biol Chem. 276: 8079-86. PMID: 11102441
5. Caspase 8 activation independent of Fas (CD95/APO-1) signaling may mediate killing of B-chronic lymphocytic leukemia cells by cytotoxic drugs or gamma radiation.  |  Jones, DT., et al. 2001. Blood. 98: 2800-7. PMID: 11675354
6. p38-MAPK signals survival by phosphorylation of caspase-8 and caspase-3 in human neutrophils.  |  Alvarado-Kristensson, M., et al. 2004. J Exp Med. 199: 449-58. PMID: 14970175
7. Activity of caspase-8 determines plasticity between cell death pathways.  |  Newton, K., et al. 2019. Nature. 575: 679-682. PMID: 31723262
8. The Fas death factor.  |  Nagata, S. and Golstein, P. 1995. Science. 267: 1449-56. PMID: 7533326
9. Contenders in FasL/TNF death signaling.  |  Cleveland, JL. and Ihle, JN. 1995. Cell. 81: 479-82. PMID: 7758103
10. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains.  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
11. FLICE is activated by association with the CD95 death-inducing signaling complex (DISC).  |  Medema, JP., et al. 1997. EMBO J. 16: 2794-804. PMID: 9184224
12. Bcl-xL functions downstream of caspase-8 to inhibit Fas- and tumor necrosis factor receptor 1-induced apoptosis of MCF7 breast carcinoma cells.  |  Srinivasan, A., et al. 1998. J Biol Chem. 273: 4523-9. PMID: 9468507

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-8 Antibody (8CSP03) has 87 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-8 Antibody (8CSP03) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-8 Antibody (8CSP03) Specifications

Alternate Product Name: CASP8

Product Clone Name: 8CSP03

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: caspase-8

Epitope Species: human

Product Reactivity/Detection: caspase-8

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 87 citations in various scientific publications

Available Conjugates/Forms for caspase-8 Antibody (8CSP03): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred caspase-8 p18 antibody is caspase-8 p18 Antibody (D-8): sc-5263.