caspase-8 p18 Antibody (E-8): sc-166320

caspase-8 p18 Antibody (E-8) is a mouse monoclonal IgG3 kappa light chain antibody that detects the p18 subunit and precursor of caspase-8 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-caspase-8 p18 antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The caspase-8 protein plays a crucial role in the apoptotic signaling pathway, acting as an initiator caspase that activates downstream effector caspases by cleaving their inactive forms. This process is vital for programmed cell death, particularly in response to signals from tumor necrosis factor receptor superfamily members such as TNFRSF1A and TNFRSF6/FAS. Caspase-8 forms a complex with the adaptor molecule FADD, leading to the formation of the death-inducing signaling complex (DISC), which is essential for its activation. The active caspase-8 protein, a heterodimer composed of the p18 and p10 subunits, is predominantly expressed in peripheral blood leukocytes, thymus, liver, and spleen, highlighting caspase-8′s importance in immune regulation. Mutations or defects in the CASP8 gene can result in caspase-8 deficiency disorder (CASP8D), a condition characterized by splenomegaly and impaired apoptosis of peripheral blood leukocytes, which may ultimately lead to immunodeficiency due to compromised activation of T lymphocytes, natural killer cells, and B lymphocytes.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-8 p18 Antibody (E-8) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-8 p18 Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-8 p18 Antibody (E-8) Specifications

Alternate Product Name: CASP8

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 221-236

Epitope Target: caspase-8 p18

Epitope Species: human

Product Reactivity/Detection: p18 subunit and precursor of caspase-8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for caspase-8 p18 Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred caspase-8 p18 antibody is caspase-8 p18 Antibody (D-8): sc-5263.