dsg3 Antibody (5G11): sc-53487

dsg3 Antibody (5G11) is a mouse monoclonal IgG1 kappa light chain antibody that detects dsg3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. dsg3 (5G11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Desmoglein 3 (dsg3) plays a crucial role in maintaining cell adhesion within the epidermis, as dsg3 is a key component of desmosomes, which are specialized cell junctions that provide structural integrity to tissues. Proper functioning of dsg3 is vital for skin health, as dysfunction can lead to pemphigus vulgaris, an autoimmune blistering disorder characterized by the production of autoantibodies against dsg3, resulting in compromised adhesion between keratinocytes. dsg3 is predominantly expressed in the lower layers of the epidermis, and dsg3 expression is often altered in various skin cancers, indicating potential roles in tumor progression and metastasis. anti-dsg3 antibody (5G11) detection across different species makes this product an invaluable tool for research into skin biology and disease mechanisms, particularly in understanding molecular pathways involved in autoimmune conditions and cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

dsg3 Antibody (5G11) References:

1. Genetic characterization of human Dsg3-specific B cells isolated by flow cytometry from the peripheral blood of patients with pemphigus vulgaris.  |  Yamagami, J., et al. 2008. J Dermatol Sci. 52: 98-107. PMID: 18586466
2. High-dose pemphigus antibodies against linear epitopes of desmoglein 3 (Dsg3) can induce acantholysis and depletion of Dsg3 from keratinocytes.  |  Cirillo, N., et al. 2009. Immunol Lett. 122: 208-13. PMID: 19200441
3. Genetic analysis of desmoglein 3 (DSG3) sequence variants in patients with pemphigus vulgaris.  |  Capon, F., et al. 2009. Br J Dermatol. 161: 1403-5. PMID: 19678820
4. Desmosome disassembly in response to pemphigus vulgaris IgG occurs in distinct phases and can be reversed by expression of exogenous Dsg3.  |  Jennings, JM., et al. 2011. J Invest Dermatol. 131: 706-18. PMID: 21160493
5. Mucosal pemphigus vulgaris anti-Dsg3 IgG is pathogenic to the oral mucosa of humanized Dsg3 mice.  |  Culton, DA., et al. 2015. J Invest Dermatol. 135: 1590-1597. PMID: 25695683
6. Evidence for Dsg3 in regulating Src signaling by competing with it for binding to caveolin-1.  |  Wan, H., et al. 2016. Data Brief. 6: 124-34. PMID: 26858977
7. Role of Dsg1- and Dsg3-Mediated Signaling in Pemphigus Autoantibody-Induced Loss of Keratinocyte Cohesion.  |  Walter, E., et al. 2019. Front Immunol. 10: 1128. PMID: 31178865
8. Bullous and verrucous lichen sclerosus with autoantibodies against both desmoglein Dsg1 and Dsg3.  |  Xiao, J., et al. 2022. Int J Dermatol. 61: e259-e261. PMID: 34859893
9. Characterization of Desmoglein 3 (DSG3) as a Sensitive and Specific Marker for Esophageal Squamous Cell Carcinoma.  |  Chi, Z., et al. 2022. Gastroenterol Res Pract. 2022: 2220940. PMID: 35251162
10. The Diversity of Serum Anti-DSG3 IgG Subclasses Has a Major Impact on Pemphigus Activity and Is Predictive of Relapses After Treatment With Rituximab.  |  Golinski, ML., et al. 2022. Front Immunol. 13: 849790. PMID: 35371083

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-dsg3 Antibody (5G11) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-dsg3 Antibody (5G11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

dsg3 Antibody (5G11) Specifications

Alternate Product Name: dsg3

Product Clone Name: 5G11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: dsg3

Epitope Species: human

Product Reactivity/Detection: dsg3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for dsg3 Antibody (5G11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred dsg3 antibody is dsg3 Antibody (5H10): sc-23912.