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β-dystroglycan Antibody (A-9): sc-165999

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Datasheets
  • β-dystroglycan Antibody (A-9) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against amino acids 831-895 mapping at the C-terminus of dystroglycan precursor of human origin
  • recommended for detection of β-dystroglycan of mouse, rat and human origin by WB, IP, IF and ELISA
  • See β-dystroglycan (4F7): sc-33702 for β-dystroglycan antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for β-dystroglycan Antibody (A-9) for WB applications. These reagents are now offered in bundles with β-dystroglycan Antibody (A-9) (see ordering information below).

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    β-dystroglycan Antibody (A-9) is a mouse monoclonal IgG1 antibody that detects β-dystroglycan in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). β-dystroglycan plays a crucial role in the structural integrity of muscle cells by forming a vital link between the extracellular matrix and the cytoskeleton, which is essential for maintaining cell shape and stability. β-dystroglycan is primarily located at the sarcolemma of muscle cells, where β-dystroglycan interacts with dystrophin to form the dystrophin-associated protein complex, a key component in muscle function and integrity. Proper localization of β-dystroglycan is critical, as disruptions in β-dystroglycan function or expression can lead to muscular dystrophies and other neuromuscular disorders. Anti-β-dystroglycan antibody (A-9) enables researchers to effectively study β-dystroglycan dynamics in various biological contexts, contributing to a deeper understanding of β-dystroglycan role in muscle physiology and pathology.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    β-dystroglycan Antibody (A-9) References:

    1. Adhesion-dependent tyrosine phosphorylation of (beta)-dystroglycan regulates its interaction with utrophin.  |  James, M., et al. 2000. J Cell Sci. 113 (Pt 10): 1717-26. PMID: 10769203
    2. Formation of multiple complexes between beta-dystroglycan and dystrophin family products.  |  Royuela, M., et al. 2003. J Muscle Res Cell Motil. 24: 387-97. PMID: 14677641
    3. Ezrin-dependent regulation of the actin cytoskeleton by beta-dystroglycan.  |  Spence, HJ., et al. 2004. Hum Mol Genet. 13: 1657-68. PMID: 15175275
    4. The effect of an ionic detergent on the natively unfolded beta-dystroglycan ectodomain and on its interaction with alpha-dystroglycan.  |  Bozzi, M., et al. 2004. Protein Sci. 13: 2437-45. PMID: 15295116
    5. Suramin affects metalloproteinase-9 activity and increases β-dystroglycan levels in the diaphragm of the dystrophin-deficient mdx mouse.  |  Taniguti, AP., et al. 2012. Muscle Nerve. 46: 810-3. PMID: 23055317
    6. Identification of Dp71 Isoforms Expressed in PC12 Cells: Subcellular Localization and Colocalization with β-Dystroglycan and α1-Syntrophin.  |  Aragón, J., et al. 2016. J Mol Neurosci. 58: 201-9. PMID: 26411569
    7. β-Dystroglycan cleavage by matrix metalloproteinase-2/-9 disturbs aquaporin-4 polarization and influences brain edema in acute cerebral ischemia.  |  Yan, W., et al. 2016. Neuroscience. 326: 141-157. PMID: 27038751
    8. Tyrosine phosphorylation as a regulator of dystrophin and beta-dystroglycan interaction: A molecular insight.  |  Abdullah, M., et al. 2020. J Mol Graph Model. 99: 107623. PMID: 32348939
    9. β-Dystroglycan Restoration and Pathology Progression in the Dystrophic mdx Mouse: Outcome and Implication of a Clinically Oriented Study with a Novel Oral Dasatinib Formulation.  |  Mantuano, P., et al. 2021. Biomolecules. 11: PMID: 34827740
    10. Decreased expression of brain beta-dystroglycan in Duchenne muscular dystrophy but not in the mdx animal model.  |  Finn, DM., et al. 1998. Biochem Biophys Res Commun. 249: 231-5. PMID: 9705863

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    β-dystroglycan Antibody (A-9)

    sc-165999
    200 µg/ml
    $316.00

    β-dystroglycan Antibody (A-9): m-IgG Fc BP-HRP Bundle

    sc-539984
    200 µg Ab; 10 µg BP
    $354.00

    β-dystroglycan Antibody (A-9): m-IgG1 BP-HRP Bundle

    sc-541830
    200 µg Ab; 20 µg BP
    $354.00

    What is the recommended fixation method for immunofluorescence staining with β-dystroglycan (A-9): sc-165999 monoclonal antibody?

    Asked by: DefinitelyNotMatt
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from Satisfactory Western Blot data ofSatisfactory Western Blot data of -dystroglycan expression in MCF7 and C6 whole cell lysates and rat skeletal muscle tissue extract . -SCBT QC
    Date published: 2014-10-09
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    β-dystroglycan Antibody (A-9) is rated 5.0 out of 5 by 1.
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