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Daxx Antibody (H-7): sc-8043

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Datasheets
  • Daxx Antibody (H-7) is a mouse monoclonal IgG2a κ Daxx antibody, cited in 26 publications, provided at 200 µg/ml
  • raised against amino acids 627-739 mapping at the C-terminus of Daxx of mouse origin
  • Daxx Antibody (H-7) is recommended for detection of Daxx of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-Daxx Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Daxx (H-7): sc-8043.
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Daxx Antibody (H-7) for WB applications. These reagents are now offered in bundles with Daxx Antibody (H-7) (see ordering information below).

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Daxx Antibody (H-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Daxx protein of mouse, rat, and human origin through western blotting, immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assay. Daxx (H-7) antibody is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Daxx plays a crucial role in the regulation of apoptosis, particularly in the context of the FAS-mediated apoptotic pathway. When the FAS receptor is activated by its ligand, activation triggers a cascade of events leading to programmed cell death, which is essential for maintaining immune system balance and tissue homeostasis. Daxx is a key player in this process, as Daxx interacts with the death domain of the FAS receptor, facilitating the activation of Jun N-terminal kinase (JNK) and promoting apoptosis. Notably, while Daxx does not possess a death domain, Daxx overexpression can enhance FAS-mediated apoptosis by activating the JNK pathway. This pathway operates alongside, yet distinctly from, the FAS-FADD-FLICE pathway, which is another critical route for apoptosis. The Daxx pathway is sensitive to the apoptotic inhibitor protein Bcl-2, highlighting unique regulatory mechanisms in cell death.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Daxx Antibody (H-7) References:

  1. Inhibition of NF-kappaB acetylation and its transcriptional activity by Daxx.  |  Park, J., et al. 2007. J Mol Biol. 368: 388-97. PMID: 17362989
  2. Anti-apoptotic effects of probucol are associated with downregulation of Daxx expression in THP-1 macrophage.  |  Su, B., et al. 2007. Cardiovasc Drugs Ther. 21: 37-45. PMID: 17370120
  3. DAXX-ATRX regulation of p53 chromatin binding and DNA damage response.  |  Gulve, N., et al. 2022. Nat Commun. 13: 5033. PMID: 36028493
  4. DAXX adds a de novo H3.3K9me3 deposition pathway to the histone chaperone network.  |  Carraro, M., et al. 2023. Mol Cell. 83: 1075-1092.e9. PMID: 36868228
  5. DAXX promotes centromeric stability independently of ATRX by preventing the accumulation of R-loop-induced DNA double-stranded breaks.  |  Pinto, LM., et al. 2024. Nucleic Acids Res. 52: 1136-1155. PMID: 38038252
  6. FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis.  |  Chinnaiyan, AM., et al. 1995. Cell. 81: 505-12. PMID: 7538907
  7. TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways.  |  Hsu, H., et al. 1996. Cell. 84: 299-308. PMID: 8565075
  8. A license to kill.  |  Fraser, A. and Evan, G. 1996. Cell. 85: 781-4. PMID: 8681372
  9. Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death.  |  Boldin, MP., et al. 1996. Cell. 85: 803-15. PMID: 8681376
  10. Apoptosis by death factor.  |  Nagata, S. 1997. Cell. 88: 355-65. PMID: 9039262
  11. Mitogen-activated protein kinase-mediated Fas apoptotic signaling pathway.  |  Goillot, E., et al. 1997. Proc Natl Acad Sci U S A. 94: 3302-7. PMID: 9096388
  12. Daxx, a novel Fas-binding protein that activates JNK and apoptosis.  |  Yang, X., et al. 1997. Cell. 89: 1067-76. PMID: 9215629

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Daxx Antibody (H-7)

sc-8043
200 µg/ml
$316.00

Daxx Antibody (H-7): m-IgG Fc BP-HRP Bundle

sc-528211
200 µg Ab; 10 µg BP
$354.00

Daxx Antibody (H-7): m-IgGκ BP-HRP Bundle

sc-520534
200 µg Ab, 40 µg BP
$354.00

Daxx Antibody (H-7): m-IgG2a BP-HRP Bundle

sc-547048
200 µg Ab; 10 µg BP
$354.00

Daxx Antibody (H-7) AC

sc-8043 AC
500 µg/ml, 25% agarose
$416.00

Daxx Antibody (H-7) HRP

sc-8043 HRP
200 µg/ml
$316.00

Daxx Antibody (H-7) FITC

sc-8043 FITC
200 µg/ml
$330.00

Daxx Antibody (H-7) PE

sc-8043 PE
200 µg/ml
$343.00

Daxx Antibody (H-7) Alexa Fluor® 488

sc-8043 AF488
200 µg/ml
$357.00

Daxx Antibody (H-7) Alexa Fluor® 546

sc-8043 AF546
200 µg/ml
$357.00

Daxx Antibody (H-7) Alexa Fluor® 594

sc-8043 AF594
200 µg/ml
$357.00

Daxx Antibody (H-7) Alexa Fluor® 647

sc-8043 AF647
200 µg/ml
$357.00

Daxx Antibody (H-7) Alexa Fluor® 680

sc-8043 AF680
200 µg/ml
$357.00

Daxx Antibody (H-7) Alexa Fluor® 790

sc-8043 AF790
200 µg/ml
$357.00

What is the recommended starting dilution for Western blot for Daxx (H-7): sc-8043?

Asked by: jenni
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-8043.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 14
Date published: 2025-03-24
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Rated 4 out of 5 by from Anti-Daxx AntibodyWorks well for detecting purified DAXX, the band is around 110~120KD.
Date published: 2022-01-06
Rated 5 out of 5 by from It worked well for IF in mouse ES cellsIt worked well for conventional IF with low background in mouse ES cells.
Date published: 2020-09-29
Rated 4 out of 5 by from Work for mouse breast cancer cell linesWork for IF and WB in mouse. The signal is clear but relatively weak.
Date published: 2018-02-20
Rated 1 out of 5 by from Daxx ab for wbI got free sample from this ab and tested with 5 different mouse cell lines and unfortunately it did not work for me.
Date published: 2017-12-07
Rated 3 out of 5 by from DaxxSignal was good, detected molecular weight less than 130 KDA in Esophageal cancer cell
Date published: 2017-11-13
Rated 5 out of 5 by from very nice qualityIt has been reported in the literature that the expression of Daxx protein in gastric cancer is very high. We used this antibody to detect the expression of Daxx. The specificity of this expression is very good. After 1:500 dilution, the target band 120kda obtained
Date published: 2017-09-16
Rated 5 out of 5 by from This antibody can get clear bands.In our lab, this antibody has good quality in Western blotting. Using 1:500 I get clear bands.
Date published: 2017-06-06
Rated 5 out of 5 by from strong signalDaxx antibody used in IF . Observed strong excellent signal.
Date published: 2017-06-03
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Daxx Antibody (H-7) is rated 4.2 out of 5 by 21.
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