Profilin-1 Antibody (C-2): sc-166191

Profilin-1 Antibody (C-2) is a mouse monoclonal IgG2b antibody that detects Profilin-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Profilin-1 plays a crucial role in regulating actin polymerization by binding to and sequestering actin monomers, which is essential for various cellular processes, including cell motility, shape, and division. Profilin-1 acts as a nucleotide exchange factor, facilitating the loading of ATP onto actin, thereby promoting its polymerization. Profilin-1 is predominantly expressed in the lung, liver, placenta, and kidney, while Profilin-2 is highly expressed in the brain and skeletal muscle, highlighting the diverse functional roles of profilins in different tissues. Profilin-1′s localization in these organs suggests involvement in critical physiological processes, such as respiratory function and cellular signaling pathways. Additionally, Profilin-1′s interaction with other proteins, such as dynamin I and synapsin in the axonal and dendritic processes of the mouse brain, indicates potential roles in synaptic function and neuronal communication. anti-Profilin-1 antibody (C-2) serves as an invaluable tool for researchers studying the dynamics of actin and its implications in health and disease, including relevance in food allergies where plant profilins are recognized as pan-allergens.

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Profilin-1 Antibody (C-2) References:

1. Profilin 1 associates with stress granules and ALS-linked mutations alter stress granule dynamics.  |  Figley, MD., et al. 2014. J Neurosci. 34: 8083-97. PMID: 24920614
2. Profilin 1-mediated cytoskeletal rearrangements regulate integrin function in mouse platelets.  |  Stritt, S., et al. 2018. Blood Adv. 2: 1040-1045. PMID: 29739775
3. Silencing profilin-1 confers protection from oxLDL injury in human vascular endothelial cells.  |  Ou, Y. and Lian, W. 2018. Int J Clin Exp Pathol. 11: 3052-3060. PMID: 31938431
4. The actin regulator profilin 1 is functionally associated with the mammalian centrosome.  |  Nejedlá, M., et al. 2021. Life Sci Alliance. 4: PMID: 33184056
5. Profilin-1; a novel regulator of DNA damage response and repair machinery in keratinocytes.  |  Lee, CJ., et al. 2021. Mol Biol Rep. 48: 1439-1452. PMID: 33590416
6. Specificity and Redundancy of Profilin 1 and 2 Function in Brain Development and Neuronal Structure.  |  Di Domenico, M., et al. 2021. Cells. 10: PMID: 34571959
7. Profilin-1 negatively controls osteoclast migration by suppressing the protrusive structures based on branched actin filaments.  |  Kajikawa, S., et al. 2022. J Bone Miner Metab. 40: 561-570. PMID: 35428898
8. Profilin 1 Induces Tumor Metastasis by Promoting Microvesicle Secretion Through the ROCK 1/p-MLC Pathway in Non-Small Cell Lung Cancer.  |  Wang, Y., et al. 2022. Front Pharmacol. 13: 890891. PMID: 35586060
9. Profilin-1 regulates DNA replication forks in a context-dependent fashion by interacting with SNF2H and BOD1L.  |  Zhu, C., et al. 2022. Nat Commun. 13: 6531. PMID: 36319634
10. The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence.  |  Ayoubi, R., et al. 2023. F1000Res. 12: 348. PMID: 37576538

The preferred Profilin-1 antibody is Profilin-1 Antibody (B-10): sc-137235.