MLH1 Antibody (Clone 14): sc-56160
- MLH1 Antibody (Clone 14) is a mouse monoclonal IgG1, cited in 2 publications, provided at 50 µg/0.5 ml
- raised against full length MLH1 of human origin
- recommended for detection of MLH1 of human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MLH1 Antibody (Clone 14). This work is in progress.
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MLH1 Antibody (Clone 14) is a mouse monoclonal IgG1 antibody that detects MLH1 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MLH1 is a crucial component of the DNA mismatch repair (MMR) system, which plays a vital role in correcting DNA replication errors, thereby maintaining genetic stability. Anti-MLH1 antibody (Clone 14) helps identify deficiencies in MLH1 that can lead to microsatellite instability and an increased risk of developing several types of cancer, particularly hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 is predominantly located in the nucleus of cells, where MLH1 participates in the repair of DNA mismatches during replication. Nuclear localization is critical because MLH1 interacts with other proteins involved in the MMR pathway, ensuring that errors are corrected promptly to prevent mutations that could lead to tumorigenesis. Expression of MLH1 is often lost in various cancers, including certain ovarian tumors, which can be attributed to gene methylation or mutations, highlighting MLH1′s importance as a biomarker for cancer diagnosis and prognosis. MLH1 monoclonal antibody (Clone 14) serves as an invaluable tool for researchers studying the mechanisms of DNA repair and the implications of MMR deficiencies in cancer biology.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MLH1 Antibody (Clone 14) References:
- Human MutL homolog (MLH1) function in DNA mismatch repair: a prospective screen for missense mutations in the ATPase domain. | Ellison, AR., et al. 2004. Nucleic Acids Res. 32: 5321-38. PMID: 15475387
- Conversion analysis for mutation detection in MLH1 and MSH2 in patients with colorectal cancer. | Casey, G., et al. 2005. JAMA. 293: 799-809. PMID: 15713769
- Cancer risks associated with germline mutations in MLH1, MSH2, and MSH6 genes in Lynch syndrome. | Bonadona, V., et al. 2011. JAMA. 305: 2304-10. PMID: 21642682
- Expression defect size among unclassified MLH1 variants determines pathogenicity in Lynch syndrome diagnosis. | Hinrichsen, I., et al. 2013. Clin Cancer Res. 19: 2432-41. PMID: 23403630
- Loss of mutL homolog-1 (MLH1) expression promotes acquisition of oncogenic and inhibitor-resistant point mutations in tyrosine kinases. | Springuel, L., et al. 2016. Cell Mol Life Sci. 73: 4739-4748. PMID: 27436342
- MLH1 Deficiency-Triggered DNA Hyperexcision by Exonuclease 1 Activates the cGAS-STING Pathway. | Guan, J., et al. 2021. Cancer Cell. 39: 109-121.e5. PMID: 33338427
- MLH1 Promotor Hypermethylation in Colorectal and Endometrial Carcinomas from Patients with Lynch Syndrome. | Helderman, NC., et al. 2024. J Mol Diagn. 26: 106-114. PMID: 38061582
- Mismatch repair protein MLH1 suppresses replicative stress in BRCA2-deficient breast tumors. | Sengodan, SK., et al. 2024. J Clin Invest. 134: PMID: 38271119
- Histone H3K9 Lactylation Confers Temozolomide Resistance in Glioblastoma via LUC7L2-Mediated MLH1 Intron Retention. | Yue, Q., et al. 2024. Adv Sci (Weinh). 11: e2309290. PMID: 38477507
- Mutant huntingtin protein induces MLH1 degradation, DNA hyperexcision, and cGAS-STING-dependent apoptosis. | Sun, X., et al. 2024. Proc Natl Acad Sci U S A. 121: e2313652121. PMID: 38498709
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MLH1 Antibody (Clone 14) | sc-56160 | 50 µg/0.5 ml | $316.00 |