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Nibrin Antibody (1D7): sc-56166

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Datasheets
  • Nibrin Antibody (1D7) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
  • raised against Nibrin of human origin
  • recommended for detection of Nibrin of mouse, rat and human origin by WB and IP
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nibrin Antibody (1D7). This work is in progress.

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    Nibrin Antibody (1D7) is a mouse monoclonal IgG1 antibody that detects Nibrin in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. Nibrin plays a crucial role in DNA damage response, particularly in double-strand break repair, by forming a complex with Mre11 and Rad50, which maintains genomic stability. Nibrin is primarily located in the nucleus, where anti-Nibrin antibody (1D7) can detect participation in MRN complex formation, a key player in DNA damage detection and repair. Nibrin′s nuclear localization enables rapid response to DNA lesions, preventing mutation accumulation that could lead to cancer. Mutations in the Nibrin gene are associated with Nijmegen breakage syndrome, a condition characterized by immunodeficiency, growth retardation, and cancer predisposition, highlighting Nibrin′s critical role in maintaining cellular integrity. Nibrin (1D7) antibody serves as an invaluable tool for researchers studying DNA repair mechanisms and Nibrin′s implications in various diseases.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Nibrin Antibody (1D7) References:

    1. Distinct functional domains of nibrin mediate Mre11 binding, focus formation, and nuclear localization.  |  Desai-Mehta, A., et al. 2001. Mol Cell Biol. 21: 2184-91. PMID: 11238951
    2. Nibrin forkhead-associated domain and breast cancer C-terminal domain are both required for nuclear focus formation and phosphorylation.  |  Cerosaletti, KM. and Concannon, P. 2003. J Biol Chem. 278: 21944-51. PMID: 12679336
    3. Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm.  |  Cerosaletti, K. and Concannon, P. 2004. J Biol Chem. 279: 38813-9. PMID: 15234984
    4. Nibrin functions in Ig class-switch recombination.  |  Kracker, S., et al. 2005. Proc Natl Acad Sci U S A. 102: 1584-9. PMID: 15668383
    5. Active role for nibrin in the kinetics of atm activation.  |  Cerosaletti, K., et al. 2006. Mol Cell Biol. 26: 1691-9. PMID: 16478990
    6. Nijmegen breakage syndrome: the clearance pathway for mutant nibrin protein is allele specific.  |  Salewsky, B., et al. 2013. Gene. 519: 217-21. PMID: 23458873
    7. Nibrin is a marker of clinical outcome in patients with advanced serous ovarian cancer treated in the phase III OVA-301 trial.  |  Monk, BJ., et al. 2014. Gynecol Oncol. 132: 176-80. PMID: 24211400
    8. The role of nibrin in doxorubicin-induced apoptosis and cell senescence in Nijmegen Breakage Syndrome patients lymphocytes.  |  Alster, O., et al. 2014. PLoS One. 9: e104964. PMID: 25119968
    9. Association of the nibrin gene (NBN) variants with breast cancer.  |  Uzunoglu, H., et al. 2016. Biomed Rep. 4: 369-373. PMID: 26998278
    10. Uterine carcinosarcoma associated with a germline nibrin (NBN) mutation.  |  Markert, T., et al. 2022. Gynecol Oncol Rep. 40: 100979. PMID: 35434237

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Nibrin Antibody (1D7)

    sc-56166
    50 µg/0.5 ml
    $316.00

    Is this antibody will be used on immunohistochemical analysis of head and neck cancers?

    Asked by: Doc muskan
    Thank you for your question. We recommend you consult the primary literature for more detailed expression information for Nibrin.
    Answered by: Technical Support
    Date published: 2019-06-16

    I am using Nibrin (1D7): sc-56166 antibody for immunoprecipitation, how much antibody should I use?

    Asked by: Randy McDonald
    Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 4 out of 5 by from Performed well in WB with mouse Nibrin transfectedPerformed well in WB with mouse Nibrin transfected 293T whole cell lysate. -SCBT QC
    Date published: 2015-04-10
    Rated 4 out of 5 by from StrongStrong, clear band detected in HeLa whole cell lysate. -SCBT QC
    Date published: 2013-05-07
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    Nibrin Antibody (1D7) is rated 4.0 out of 5 by 2.
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