XRCC4 Antibody (C-4): sc-271087

XRCC4 Antibody (C-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects XRCC4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-XRCC4 antibody (C-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. XRCC4 plays a crucial role in the DNA double-strand break repair (DSBR) pathway, which is essential for maintaining genomic stability and preventing chromosomal aberrations that can lead to cancer. Located primarily in the nucleus, XRCC4 interacts with other key proteins involved in DNA repair, such as DNA ligase IV, to facilitate the ligation of DNA ends during the repair process. This interaction is vital because any disruption in the DSBR pathway can result in severe consequences, including increased susceptibility to genomic instability and cancer development. XRCC4 has been shown to restore DSBR activity and V(D)J recombination in cells with impaired repair mechanisms, highlighting its importance in both somatic and immune system development. XRCC4′s ability to function effectively in these processes underscores its significance as a target for research into DNA repair mechanisms and potential therapeutic interventions in cancer.

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XRCC4 Antibody (C-4) References:

1. DNA binding of Xrcc4 protein is associated with V(D)J recombination but not with stimulation of DNA ligase IV activity.  |  Modesti, M., et al. 1999. EMBO J. 18: 2008-18. PMID: 10202163
2. Mammalian XRCC2 promotes the repair of DNA double-strand breaks by homologous recombination.  |  Johnson, RD., et al. 1999. Nature. 401: 397-9. PMID: 10517641
3. XRCC3 promotes homology-directed repair of DNA damage in mammalian cells.  |  Pierce, AJ., et al. 1999. Genes Dev. 13: 2633-8. PMID: 10541549
4. Processing of DNA for nonhomologous end-joining is controlled by kinase activity and XRCC4/ligase IV.  |  Budman, J., et al. 2007. J Biol Chem. 282: 11950-9. PMID: 17272270
5. XRCC4:DNA ligase IV can ligate incompatible DNA ends and can ligate across gaps.  |  Gu, J., et al. 2007. EMBO J. 26: 1010-23. PMID: 17290226
6. Knockdown of DNA ligase IV/XRCC4 by RNA interference inhibits herpes simplex virus type I DNA replication.  |  Muylaert, I. and Elias, P. 2007. J Biol Chem. 282: 10865-72. PMID: 17296606
7. Length-dependent binding of human XLF to DNA and stimulation of XRCC4.DNA ligase IV activity.  |  Lu, H., et al. 2007. J Biol Chem. 282: 11155-62. PMID: 17317666
8. Curcumin Induces Apoptosis by Suppressing XRCC4 Expression in Hepatocellular Carcinoma.  |  Wang, S., et al. 2023. Nutr Cancer. 75: 1958-1967. PMID: 37899756
9. Multivalent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro.  |  Vu, DD., et al. 2024. Nat Struct Mol Biol. 31: 1732-1744. PMID: 38898102
10. Identification of the XRCC4 gene: complementation of the DSBR and V(D)J recombination defects of XR-1 cells.  |  Li, Z. and Alt, FW. 1996. Curr Top Microbiol Immunol. 217: 143-50. PMID: 8787623
11. XRCC1 protein interacts with one of two distinct forms of DNA ligase III.  |  Nash, RA., et al. 1997. Biochemistry. 36: 5207-11. PMID: 9136882
12. XRCC2 and XRCC3, new human Rad51-family members, promote chromosome stability and protect against DNA cross-links and other damages.  |  Liu, N., et al. 1998. Mol Cell. 1: 783-93. PMID: 9660962

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-XRCC4 Antibody (C-4) has 29 citations in a variety of scientific publications.

Primary antibodies like Anti-XRCC4 Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

XRCC4 Antibody (C-4) Specifications

Alternate Product Name: XRCC4

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-233

Epitope Target: XRCC4

Epitope Species: human

Product Reactivity/Detection: XRCC4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 29 citations in various scientific publications

Available Conjugates/Forms for XRCC4 Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin