BUBR1 Antibody (8G1): sc-47744

BUBR1 Antibody (8G1) is a mouse monoclonal IgG1 kappa light chain antibody that detects BUBR1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-BUBR1 antibody (8G1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BUBR1 plays a critical role in the mitotic checkpoint, ensuring that chromosomes are properly aligned before cell division proceeds. BUBR1 (8G1) monoclonal antibody localizes to the kinetochore, where BUBR1 interacts with microtubules to monitor their attachment and stability. This localization prevents the premature separation of sister chromatids, safeguarding genomic integrity during cell division. Disruption of BUBR1 function can lead to aneuploidy and has been implicated in various cancers, highlighting BUBR1′s importance in maintaining proper cell cycle regulation. BUBR1 interacts with other key proteins such as BUB3, which is essential for the spindle assembly checkpoint, further underscoring BUBR1′s role in the complex network of proteins that govern mitosis.

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BUBR1 Antibody (8G1) References:

1. Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC.  |  Chan, GK., et al. 1999. J Cell Biol. 146: 941-54. PMID: 10477750
2. BUBR1 phosphorylation is regulated during mitotic checkpoint activation.  |  Li, W., et al. 1999. Cell Growth Differ. 10: 769-75. PMID: 10593653
3. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis.  |  Kalitsis, P., et al. 2000. Genes Dev. 14: 2277-82. PMID: 10995385
4. CENP-E as an essential component of the mitotic checkpoint in vitro.  |  Abrieu, A., et al. 2000. Cell. 102: 817-26. PMID: 11030625
5. BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice.  |  Baker, DJ., et al. 2004. Nat Genet. 36: 744-9. PMID: 15208629
6. BubR1 insufficiency inhibits neointimal hyperplasia through impaired vascular smooth muscle cell proliferation in mice.  |  Kyuragi, R., et al. 2015. Arterioscler Thromb Vasc Biol. 35: 341-7. PMID: 25524773
7. BubR1 alterations that reinforce mitotic surveillance act against aneuploidy and cancer.  |  Weaver, RL., et al. 2016. Elife. 5: PMID: 27528194
8. BubR1 recruitment to the kinetochore via Bub1 enhances spindle assembly checkpoint signaling.  |  Banerjee, A., et al. 2022. Mol Biol Cell. 33: br16. PMID: 35767360
9. BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma.  |  Delgado, L., et al. 2022. Animals (Basel). 12: PMID: 36428310
10. A conserved CENP-E region mediates BubR1-independent recruitment to the outer corona at mitotic onset.  |  Weber, J., et al. 2024. Curr Biol. 34: 1133-1141.e4. PMID: 38354735
11. Identification of a novel gene--SSK1--in human endothelial cells exposed to shear stress.  |  Donadelli, R., et al. 1998. Biochem Biophys Res Commun. 246: 881-7. PMID: 9618306
12. The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosis.  |  Jablonski, SA., et al. 1998. Chromosoma. 107: 386-96. PMID: 9914370

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BUBR1 Antibody (8G1) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-BUBR1 Antibody (8G1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BUBR1 Antibody (8G1) Specifications

Alternate Product Name: BUBR1

Product Clone Name: 8G1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-350

Epitope Target: BUBR1

Epitope Species: human

Product Reactivity/Detection: BUBR1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for BUBR1 Antibody (8G1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin