Cdc5L Antibody (D-11): sc-398280

Cdc5L Antibody (D-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cdc5L protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Cdc5L antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cdc5L protein, also known as Cell division cycle 5-like protein, plays a crucial role in cell cycle regulation and is essential for pre-mRNA splicing, a process vital for proper gene expression. Cdc5L contains two helix-turn-helix myb-type DNA-binding domains, allowing DNA interaction and participation in the second catalytic step of pre-mRNA splicing, which involves cleavage at the 3′ splice site and exon ligation, ultimately releasing the intact intron lariat. This function ensures accurate mRNA processing, critical for functional protein synthesis. Cdc5L is a highly conserved spliceosomal protein forming part of core spliceosomal complexes, underscoring evolutionary significance. Notably, chromosomal aberrations involving Cdc5L have been implicated in multicystic renal dysplasia, highlighting relevance in human disease.

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Cdc5L Antibody (D-11) References:

1. Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry.  |  Ajuh, P., et al. 2000. EMBO J. 19: 6569-81. PMID: 11101529
2. A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing.  |  Ajuh, P., et al. 2001. J Biol Chem. 276: 42370-81. PMID: 11544257
3. Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.  |  Jurica, MS., et al. 2002. RNA. 8: 426-39. PMID: 11991638
4. hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing.  |  Leonard, D., et al. 2003. Biochem Biophys Res Commun. 308: 793-801. PMID: 12927788
5. Identification of peptide inhibitors of pre-mRNA splicing derived from the essential interaction domains of CDC5L and PLRG1.  |  Ajuh, P. and Lamond, AI. 2003. Nucleic Acids Res. 31: 6104-16. PMID: 14576297
6. Cell cycle regulator gene CDC5L, a potential target for 6p12-p21 amplicon in osteosarcoma.  |  Lu, XY., et al. 2008. Mol Cancer Res. 6: 937-46. PMID: 18567798
7. Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice.  |  Llères, D., et al. 2010. EMBO Rep. 11: 445-51. PMID: 20467437
8. Editor's Note to 'Identification of peptide inhibitors of pre-mRNA splicing derived from the essential interaction domains of CDC5L and PLRG1'.  |  . 2022. Nucleic Acids Res. 50: 8400. PMID: 35848918
9. BCAS2 regulates oocyte meiotic prophase I by participating in mRNA alternative splicing.  |  Yao, X., et al. 2024. FASEB J. 38: e23361. PMID: 38085152
10. A cdc5+ homolog of a higher plant, Arabidopsis thaliana.  |  Hirayama, T. and Shinozaki, K. 1996. Proc Natl Acad Sci U S A. 93: 13371-6. PMID: 8917598
11. Pombe Cdc5-related protein. A putative human transcription factor implicated in mitogen-activated signaling.  |  Bernstein, HS. and Coughlin, SR. 1997. J Biol Chem. 272: 5833-7. PMID: 9038199
12. Rearrangement of the human CDC5L gene by a t(6;19)(p21;q13.1) in a patient with multicystic renal dysplasia.  |  Groenen, PM., et al. 1998. Genomics. 49: 218-29. PMID: 9598309

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc5L Antibody (D-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc5L Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc5L Antibody (D-11) Specifications

Alternate Product Name: Cdc5L

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 503-802

Epitope Target: Cdc5L

Epitope Species: human

Product Reactivity/Detection: Cdc5L

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Cdc5L Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin