Cdc25A Antibody (F-6): sc-7389
- Cdc25A Antibody (F-6) is a mouse monoclonal IgG1 κ Cdc25A antibody, cited in 245 publications, provided at 200 µg/ml
- epitope mapping at the C-terminus of Cdc25A of mouse origin
- Cdc25A Antibody (F-6) is recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP and IF
- Anti-Cdc25A Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc25A (F-6): sc-7389.
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Cdc25A Antibody (F-6) for WB applications. This reagent is now offered in a bundle with Cdc25A Antibody (F-6) (see ordering information below).
Cdc25A Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cdc25A of mouse, rat, and human origin through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Cdc25A monoclonal antibody (F-6) is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cdc25A plays a crucial role in cell cycle regulation, specifically in the transition from G2 phase to mitosis. Cdc25A acts as a phosphatase that dephosphorylates cyclin-dependent kinase Cdc2, thereby activating the Cdc2/cyclin B complex, which is essential for mitosis initiation. Precise regulation of Cdc25A is vital, as overexpression can lead to uncontrolled cell division and has been implicated in various cancers. Cdc25A activity is tightly controlled by post-translational modifications, including phosphorylation, which can either activate or inhibit function, highlighting the importance of understanding regulatory mechanisms in cell cycle progression and tumorigenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc25A Antibody (F-6) References:
- Clues to action of cdc25 protein. | Moreno, S. and Nurse, P. 1991. Nature. 351: 194. PMID: 1645849
- Distinct nuclear and spindle pole body population of cyclin-cdc2 in fission yeast. | Alfa, CE., et al. 1990. Nature. 347: 680-2. PMID: 1699136
- Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins. | Galaktionov, K. and Beach, D. 1991. Cell. 67: 1181-94. PMID: 1836978
- cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2. | Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
- Control of M-phase by maturation-promoting factor. | Dorée, M. 1990. Curr Opin Cell Biol. 2: 269-73. PMID: 2141993
- Direct activation of cdc2 with phosphatase: identification of p13suc1-sensitive and insensitive steps. | Jessus, C., et al. 1990. FEBS Lett. 266: 4-8. PMID: 2163887
- Regulation of mitosis by cyclic accumulation of p80cdc25 mitotic inducer in fission yeast. | Moreno, S., et al. 1990. Nature. 344: 549-52. PMID: 2320127
- Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis. | Gould, KL. and Nurse, P. 1989. Nature. 342: 39-45. PMID: 2682257
- Dominoes and clocks: the union of two views of the cell cycle. | Murray, AW. and Kirschner, MW. 1989. Science. 246: 614-21. PMID: 2683077
- Cdc25A inhibits autophagy-mediated ferroptosis by upregulating ErbB2 through PKM2 dephosphorylation in cervical cancer cells. | Wang, C., et al. 2021. Cell Death Dis. 12: 1055. PMID: 34743185
- SRSF10 stabilizes CDC25A by triggering exon 6 skipping to promote hepatocarcinogenesis. | Liu, X., et al. 2022. J Exp Clin Cancer Res. 41: 353. PMID: 36539837
- CHK1-CDC25A-CDK1 regulate cell cycle progression and protect genome integrity in early mouse embryos. | Knoblochova, L., et al. 2023. EMBO Rep. 24: e56530. PMID: 37694680
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc25A Antibody (F-6) | sc-7389 | 200 µg/ml | $316.00 | |||
Cdc25A Antibody (F-6): m-IgG1 BP-HRP Bundle | sc-531629 | 200 µg Ab; 20 µg BP | $354.00 | |||
Cdc25A Antibody (F-6) AC | sc-7389 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Cdc25A Antibody (F-6) HRP | sc-7389 HRP | 200 µg/ml | $316.00 | |||
Cdc25A Antibody (F-6) FITC | sc-7389 FITC | 200 µg/ml | $330.00 | |||
Cdc25A Antibody (F-6) PE | sc-7389 PE | 200 µg/ml | $343.00 | |||
Cdc25A Antibody (F-6) Alexa Fluor® 488 | sc-7389 AF488 | 200 µg/ml | $357.00 | |||
Cdc25A Antibody (F-6) Alexa Fluor® 546 | sc-7389 AF546 | 200 µg/ml | $357.00 | |||
Cdc25A Antibody (F-6) Alexa Fluor® 594 | sc-7389 AF594 | 200 µg/ml | $357.00 | |||
Cdc25A Antibody (F-6) Alexa Fluor® 647 | sc-7389 AF647 | 200 µg/ml | $357.00 | |||
Cdc25A Antibody (F-6) Alexa Fluor® 680 | sc-7389 AF680 | 200 µg/ml | $357.00 | |||
Cdc25A Antibody (F-6) Alexa Fluor® 790 | sc-7389 AF790 | 200 µg/ml | $357.00 |