Cdc27 Antibody (B-4): sc-376964

Cdc27 Antibody (B-4) is a mouse monoclonal IgG1 antibody that detects Cdc27 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cdc27 plays a crucial role in the regulation of the cell cycle, particularly as a component of the anaphase-promoting complex (APC), which is essential for the timely degradation of cyclins and other cell cycle regulators. This degradation is vital for the transition from metaphase to anaphase during cell division, ensuring that cells do not prematurely enter the next phase of the cycle. The proper functioning of Cdc27 is critical for maintaining genomic stability, as dysregulation can lead to uncontrolled cell proliferation and is often implicated in various cancers. Anti-Cdc27 antibody (B-4) enables researchers to effectively study the dynamics of cell cycle regulation and the implications of Cdc27 in cellular processes, providing insights into potential therapeutic targets for cancer treatment.

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Cdc27 Antibody (B-4) References:

1. Essential interaction between the fission yeast DNA polymerase delta subunit Cdc27 and Pcn1 (PCNA) mediated through a C-terminal p21(Cip1)-like PCNA binding motif.  |  Reynolds, N., et al. 2000. EMBO J. 19: 1108-18. PMID: 10698951
2. Non-cell-autonomous rescue of anaphase-promoting complex function revealed by mosaic analysis of HOBBIT, an Arabidopsis CDC27 homolog.  |  Serralbo, O., et al. 2006. Proc Natl Acad Sci U S A. 103: 13250-5. PMID: 16938844
3. Molecular structure of the N-terminal domain of the APC/C subunit Cdc27 reveals a homo-dimeric tetratricopeptide repeat architecture.  |  Zhang, Z., et al. 2010. J Mol Biol. 397: 1316-28. PMID: 20206185
4. The APC/C subunit Cdc16/Cut9 is a contiguous tetratricopeptide repeat superhelix with a homo-dimer interface similar to Cdc27.  |  Zhang, Z., et al. 2010. EMBO J. 29: 3733-44. PMID: 20924356
5. Molecular basis for the association of microcephalin (MCPH1) protein with the cell division cycle protein 27 (Cdc27) subunit of the anaphase-promoting complex.  |  Singh, N., et al. 2012. J Biol Chem. 287: 2854-62. PMID: 22139841
6. CDC27 protein is involved in radiation response in squamous cell cervix carcinoma.  |  Rajkumar, T., et al. 2005. Indian J Biochem Biophys. 42: 271-8. PMID: 23923534
7. Comprehensive Analysis of CDC27 Related to Peritoneal Metastasis by Whole Exome Sequencing in Gastric Cancer.  |  Wu, R., et al. 2020. Onco Targets Ther. 13: 3335-3346. PMID: 32368092
8. Implications of ITCH-mediated ubiquitination of SIX1 on CDC27-cyclinB1 signaling in nasopharyngeal carcinoma.  |  Lin, Z., et al. 2024. Sci Rep. 14: 24140. PMID: 39406717
9. The fission yeast Cdc1 protein, a homologue of the small subunit of DNA polymerase delta, binds to Pol3 and Cdc27.  |  MacNeill, SA., et al. 1996. EMBO J. 15: 4613-28. PMID: 8887553
10. The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis.  |  Prinz, S., et al. 1998. Curr Biol. 8: 750-60. PMID: 9651679

The preferred Cdc27 antibody is Cdc27 Antibody (AF3.1): sc-9972.