Cdc27 Antibody (D-2): sc-376688
- Cdc27 Antibody (D-2) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-29 at the N-terminus of Cdc27 of human origin
- recommended for detection of Cdc27 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- See Cdc27 (AF3.1): sc-9972 for Cdc27 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc27 Antibody (D-2) for WB applications. These reagents are now offered in bundles with Cdc27 Antibody (D-2) (see ordering information below).
Cdc27 Antibody (D-2) is a mouse monoclonal IgG2b light chain antibody that detects Cdc27 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cdc27 plays a crucial role in the regulation of the cell cycle, particularly as a component of the anaphase-promoting complex (APC), which is essential for the timely degradation of cyclins and other cell cycle regulators. This degradation is vital for the transition from metaphase to anaphase during cell division, ensuring that cells do not prematurely enter the next phase of the cycle. The proper functioning of Cdc27 is critical for maintaining genomic stability, as dysregulation can lead to uncontrolled cell proliferation and is often implicated in various cancers. Anti-Cdc27 antibody (D-2) provides a reliable tool for researchers studying cell cycle dynamics and the molecular mechanisms underlying cell division, making this resource invaluable for advancing our understanding of cellular processes and their implications in disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc27 Antibody (D-2) References:
- The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta. | Bermudez, VP., et al. 2002. J Biol Chem. 277: 36853-62. PMID: 12124382
- Depletion of anaphase-promoting complex or cyclosome (APC/C) subunit homolog APC1 or CDC27 of Trypanosoma brucei arrests the procyclic form in metaphase but the bloodstream form in anaphase. | Kumar, P. and Wang, CC. 2005. J Biol Chem. 280: 31783-91. PMID: 15994309
- C/EBP{delta} targets cyclin D1 for proteasome-mediated degradation via induction of CDC27/APC3 expression. | Pawar, SA., et al. 2010. Proc Natl Acad Sci U S A. 107: 9210-5. PMID: 20439707
- Low cdc27 and high securin expression predict short survival for breast cancer patients. | Talvinen, K., et al. 2013. APMIS. 121: 945-53. PMID: 23755904
- Hsa_circ_0044226 knockdown attenuates progression of pulmonary fibrosis by inhibiting CDC27. | Qi, F., et al. 2020. Aging (Albany NY). 12: 14808-14818. PMID: 32710728
- Prognostic and clinical significance of subcellular CDC27 for patients with rectal adenocarcinoma treated with adjuvant chemotherapy. | Chang, CL., et al. 2022. Oncol Lett. 24: 238. PMID: 35720473
- Nuclear-localized CTEN is a novel transcriptional regulator and promotes cancer cell migration through its downstream target CDC27. | Wang, YX., et al. 2023. J Physiol Biochem. 79: 163-174. PMID: 36399312
- Interleukin 27 is a novel cytokine with anti-inflammatory effects against spondyloarthritis through the suppression of Th17 responses. | Jouhault, Q., et al. 2022. Front Immunol. 13: 1072420. PMID: 36818477
- Localization of the human homolog of the yeast cell division control 27 gene (CDC27) proximal to ITGB3 on human chromosome 17q21.3. | Ho, PP., et al. 1995. Somat Cell Mol Genet. 21: 351-5. PMID: 8619132
- The yeast CDC16 and CDC27 genes restrict DNA replication to once per cell cycle. | Heichman, KA. and Roberts, JM. 1996. Cell. 85: 39-48. PMID: 8620535
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc27 Antibody (D-2) | sc-376688 | 200 µg/ml | $316.00 | |||
Cdc27 Antibody (D-2): m-IgGκ BP-HRP Bundle | sc-535505 | 200 µg Ab; 40 µg BP | $354.00 | |||
Cdc27 Antibody (D-2): m-IgG2b BP-HRP Bundle | sc-549434 | 200 µg Ab; 10 µg BP | $354.00 | |||
Cdc27 (D-2) Neutralizing Peptide | sc-376688 P | 100 µg/0.5 ml | $68.00 |