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Cdk4 Antibody (DCS-31): sc-56277

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Datasheets
  • Cdk4 Antibody (DCS-31) is a mouse monoclonal IgG2a κ, cited in 52 publications, provided at 200 µg/ml
  • raised against full length Cdk4 of human origin
  • Anti-Cdk4 Antibody (DCS-31) is recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP, IF and ELISA; non cross-reactive with other Cdk types
  • Anti-Cdk4 Antibody (DCS-31) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdk4 (DCS-31): sc-56277.
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdk4 Antibody (DCS-31) for WB applications. These reagents are now offered in bundles with Cdk4 Antibody (DCS-31) (see ordering information below).

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Cdk4 Antibody (DCS-31) is a mouse monoclonal IgG2a kappa light chain antibody specifically engineered to detect Cyclin-dependent kinase 4 (Cdk4), also known as CDC2-like kinase 4, in mouse, rat, and human samples. anti-Cdk4 antibody (DCS-31) recognizes the full-length human Cdk4 protein, making it suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cdk4 is a pivotal kinase located primarily in the nucleus and cytoplasm, where it forms complexes with D-type cyclins to regulate the progression of the cell cycle from the G1 to the S phase. By phosphorylating key substrates like the retinoblastoma protein (Rb), Cdk4 controls essential transitions that drive cell division, underscoring its importance in cellular proliferation and cancer research. The activity of Cdk4 is finely tuned through interactions with cyclins, which enhance its kinase activity, and inhibitors such as p16, which suppress its function. Additionally, the activation of Cdk4-cyclin complexes involves phosphorylation by Cdk-activating proteins (CAK), highlighting the sophisticated regulatory mechanisms that ensure proper cell cycle progression. Cdk4 monoclonal antibody (DCS-31) is available in both non-conjugated form and a variety of conjugated formats, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, to accommodate diverse experimental needs. Cdk4 (DCS-31) antibody is an invaluable tool for researchers investigating cell cycle dynamics and related signaling pathways.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Cdk4 Antibody (DCS-31)

sc-56277
200 µg/ml
$316.00

Cdk4 Antibody (DCS-31): m-IgG Fc BP-HRP Bundle

sc-528616
200 µg Ab; 10 µg BP
$354.00

Cdk4 Antibody (DCS-31): m-IgGκ BP-HRP Bundle

sc-521037
200 µg Ab, 40 µg BP
$354.00

Cdk4 Antibody (DCS-31) AC

sc-56277 AC
500 µg/ml, 25% agarose
$416.00

Cdk4 Antibody (DCS-31) HRP

sc-56277 HRP
200 µg/ml
$316.00

Cdk4 Antibody (DCS-31) FITC

sc-56277 FITC
200 µg/ml
$330.00

Cdk4 Antibody (DCS-31) PE

sc-56277 PE
200 µg/ml
$343.00

Cdk4 Antibody (DCS-31) Alexa Fluor® 488

sc-56277 AF488
200 µg/ml
$357.00

Cdk4 Antibody (DCS-31) Alexa Fluor® 546

sc-56277 AF546
200 µg/ml
$357.00

Cdk4 Antibody (DCS-31) Alexa Fluor® 594

sc-56277 AF594
200 µg/ml
$357.00

Cdk4 Antibody (DCS-31) Alexa Fluor® 647

sc-56277 AF647
200 µg/ml
$357.00

Cdk4 Antibody (DCS-31) Alexa Fluor® 680

sc-56277 AF680
200 µg/ml
$357.00

Cdk4 Antibody (DCS-31) Alexa Fluor® 790

sc-56277 AF790
200 µg/ml
$357.00

I need it for IP experiment. What is the specific protocol for this antibody conjugated to agarose beads?

Asked by: Anonymous
Thank you for your question. You can find our recommended IP protocol by following this link: https://www.scbt.com/resources/protocols/immunoprecipitation-western-blots
Answered by: Tech Support Europe
Date published: 2020-09-07

I work primarily with zebrafish samples. Will this antibody work for me?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 1
Date published: 2017-01-13

I don't have any more of my donkey anti-mouse secondary. What can I use instead?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 1
Date published: 2017-01-13
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Rated 4 out of 5 by from Anticorpo Cdk4Utilizzato in saggi di Western Blot, ha funzionato discretamente.
Date published: 2020-11-06
Rated 5 out of 5 by from Great anitbody!Very specific! no background! used 1:1000 dilution
Date published: 2018-03-01
Rated 5 out of 5 by from Great antibody for western blotThe batch of antibody I have worked well on BJAB, HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 250 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2018-01-04
Rated 5 out of 5 by from Great antibody for IB!This is a great antibody for IB, quite clean signal with little background. Better than sc-23896.
Date published: 2017-11-28
Rated 5 out of 5 by from Good antibody!I like this antibody as it has strong band binding and no background.
Date published: 2017-10-13
Rated 5 out of 5 by from It works excellent in WBThis antibody works excellent in WB. Great antibody.
Date published: 2017-09-20
Rated 5 out of 5 by from GOOD FOR IB, IPI tested this antibody to IB(1:100), and IP (2ug/ml lysated protein) in HepG2, PLC/PRF/5, Huh7 and BT-549 cells. It recognizes the endogenous CDK4 protein, and also works for IP.
Date published: 2017-07-10
Rated 5 out of 5 by from WBclear band ,claen background and accurate protein position
Date published: 2017-06-05
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Cdk4 Antibody (DCS-31) is rated 4.9 out of 5 by 15.
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