cyclin D1 Antibody (DCS-6): sc-20044

cyclin D1 Antibody (DCS-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects cyclin D1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). cyclin D1 Antibody (DCS-6) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. cyclin D1 plays a pivotal role in cell cycle regulation, particularly in the transition from G1 phase to S phase, where cyclin D1 is essential for cell progression into DNA synthesis. This regulation is crucial because improper control of the cell cycle can lead to uncontrolled cell proliferation, a hallmark of cancer. cyclin D1 interacts with cyclin-dependent kinases (CDKs) to phosphorylate the retinoblastoma protein, which releases E2F transcription factors that drive the expression of genes necessary for S phase entry. Dysregulation of cyclin D1 has been implicated in various malignancies, making cyclin D1 a significant focus of cancer research. Understanding cyclin D1 function and regulation is vital for developing targeted therapies and improving cancer treatment outcomes.

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cyclin D1 Antibody (DCS-6) References:

1. Cloning of a D-type cyclin from murine erythroleukemia cells.  |  Kiyokawa, H., et al. 1992. Proc Natl Acad Sci U S A. 89: 2444-7. PMID: 1372445
2. Cloning and characterization of human cyclin D3, a cDNA closely related in sequence to the PRAD1/cyclin D1 proto-oncogene.  |  Motokura, T., et al. 1992. J Biol Chem. 267: 20412-5. PMID: 1383201
3. Genomic organization, chromosomal localization, and independent expression of human cyclin D genes.  |  Inaba, T., et al. 1992. Genomics. 13: 565-74. PMID: 1386335
4. Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins.  |  Xiong, Y., et al. 1992. Genomics. 13: 575-84. PMID: 1386336
5. Growth-regulated expression of D-type cyclin genes in human diploid fibroblasts.  |  Won, KA., et al. 1992. Proc Natl Acad Sci U S A. 89: 9910-4. PMID: 1409718
6. Human D-type cyclin.  |  Xiong, Y., et al. 1991. Cell. 65: 691-9. PMID: 1827756
7. Cell cycle control in eukaryotes: molecular mechanisms of cdc2 activation.  |  Draetta, G. 1990. Trends Biochem Sci. 15: 378-83. PMID: 2147521
8. Functional interactions of the retinoblastoma protein with mammalian D-type cyclins.  |  Ewen, ME., et al. 1993. Cell. 73: 487-97. PMID: 8343202
9. Physical interaction of the retinoblastoma protein with human D cyclins.  |  Dowdy, SF., et al. 1993. Cell. 73: 499-511. PMID: 8490963

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cyclin D1 Antibody (DCS-6) has 453 citations in a variety of scientific publications.

Primary antibodies like Anti-cyclin D1 Antibody (DCS-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cyclin D1 Antibody (DCS-6) Specifications

Alternate Product Name: cyclin D1

Product Clone Name: DCS-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: cyclin D1

Epitope Species: human

Product Reactivity/Detection: cyclin D1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 453 citations in various scientific publications

Available Conjugates/Forms for cyclin D1 Antibody (DCS-6): AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin