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215642 Goat Anti-MTHFD1L, aa535-538 (FTHFSDC1, 10-Formyl-THF Synthetase, MTC1THFS)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Swiss Prot
Q6UB35
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu Mo Rt
Accession #
NP_056255.2
Gene ID
25902
Gene #
FTHFSDC1 / MTHFD1L
Shipping Temp
Blue Ice
Storage Temp
-20°C

May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2 By similarity.

Applications
Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:32,000 Western Blot: 0.1-0.3ug/ml, ~110kD band observed in human brain (cerebellum) Immunohistochemistry (Paraffin): 5ug/ml. Antigen retrieval with citrate buffer, pH 6.0 is required. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-DTNDRFLRKITIGQ from an internal region of human MTHFD1L. Species Crossreactivity: canine, porcine and bovine
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human MTHFD1L. Species Crossreactivity: mouse and rat
References
1. Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen|A high-throughput pipeline for validation of antibodies Nat Methods. 2018 Nov; 15(11):909-912 PMID: 30377371
USBio References
No references available
Conjugates
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