MTHFD1L, aa535-538

Clone Type

Polyclonal

Host

Goat

Source

Human

Swiss Prot

Q6UB35

Isotype

IgG

Grade

Affinity Purified

Applications

E IHC WB

Crossreactivity

Hu Mo Rt

Accession #

NP_056255.2

Gene ID

25902

Gene #

FTHFSDC1 / MTHFD1L

Shipping Temp

Blue Ice

Storage Temp

-20°C

May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2 By similarity.

Applications

Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution

Peptide ELISA: 1:32,000 Western Blot: 0.1-0.3ug/ml, ~110kD band observed in human brain (cerebellum) Immunohistochemistry (Paraffin): 5ug/ml. Antigen retrieval with citrate buffer, pH 6.0 is required. Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to C-DTNDRFLRKITIGQ from an internal region of human MTHFD1L. Species Crossreactivity: canine, porcine and bovine

Form

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human MTHFD1L. Species Crossreactivity: mouse and rat

References

1. Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen|A high-throughput pipeline for validation of antibodies Nat Methods. 2018 Nov; 15(11):909-912 PMID: 30377371

USBio References

No references available

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215642 Goat Anti-MTHFD1L, aa535-538 (FTHFSDC1, 10-Formyl-THF Synthetase, MTC1THFS)

Clone Type

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Crossreactivity

Accession #

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References

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