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Human MMP-1/TIMP-1 Complex DuoSet ELISA

Catalog #: DY1550
4 Citations Datasheet / COA / SDS

Discontinued Product

DY1550 has been discontinued.
View all MMP-1/TIMP-1 Complex products.
Human MMP-1 / TIMP-1 Complex ELISA Standard Curve
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Product Details
Citations (4)
FAQs
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage

Human MMP-1/TIMP-1 Complex DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
62.5 - 4,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human MMP-1 / TIMP-1 Complex. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Human MMP-1 / TIMP-1 Complex ELISA Standard Curve View Larger

Human MMP-1 / TIMP-1 Complex ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Human MMP-1/TIMP-1 Complex DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. siRNA knockdown of tissue inhibitor of metalloproteinase-1 in keloid fibroblasts leads to degradation of collagen type I.
    Authors: Aoki, Masayo, Miyake, Koichi, Ogawa, Rei, Dohi, Teruyuki, Akaishi, Satoshi, Hyakusoku, Hiko, Shimada, Takashi
    J Invest Dermatol, 2013-09-16;134(3):818-26.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Adiponectin inhibits leptin signalling via multiple mechanisms to exert protective effects against hepatic fibrosis.
    Authors: Handy JA, Fu PP, Kumar P
    Biochem. J., 2011-12-15;440(3):385-95.
    Species: Rat
    Sample Types: Cell Culture Supernates
  3. Serum levels of sex steroid hormones and matrix metalloproteinases after intra-articular glucocorticoid treatment in female patients with rheumatoid arthritis.
    Authors: Weitoft T, Larsson A, Ronnblom L
    Ann. Rheum. Dis., 2007-09-18;67(3):422-4.
    Species: Human
    Sample Types: Serum
  4. Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
    Authors: Naveau A, Reinald N, Fournier B
    Connect. Tissue Res., 2007-01-01;48(6):300-8.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

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