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Mouse CD3+ T Cell Enrichment Column, Small, Pack of 5

Catalog #: MTCC-5
18 Citations Datasheet / COA / SDS

Discontinued Product

MTCC-5 has been discontinued.
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R&D Systems Cell Enrichment Column Kits: Antibody-coated Silica Beads
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Mouse CD3+ T Cell Enrichment Column, Small, Pack of 5 Summary

T Cell Enrichment Columns

T Cell Enrichment Columns Assay Principle
View Full Assay Principle

Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.

CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 45% and 65% of initial CD3+ cells loaded. The purity of recovered cells ranges from 81% to 88%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.

Features

  • Available for human, mouse, and rat cells
  • Purifies CD3+ T cells to 81 – 88%
  • Based upon gravitational flow
  • Simple procedure
  • No specialized equipment required

Kit Contents

  • Cell Selection Column
  • 10X Column Buffer

Specifications

Source
N/A
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Mouse

Product Datasheets

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Background: CD3

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Entrez Gene IDs
916 (Human); 12501 (Mouse)
Alternate Names
CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Citations for Mouse CD3+ T Cell Enrichment Column, Small, Pack of 5

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. An Igh distal enhancer modulates antigen receptor diversity by determining locus conformation
    Authors: KH Bhat, S Priyadarsh, S Naiyer, X Qu, H Farooq, E Kleiman, J Xu, X Lei, JF Cantillo, R Wuerffel, N Baumgarth, J Liang, AJ Feeney, AL Kenter
    Nature Communications, 2023-03-03;14(1):1225.  2023-03-03
  2. An Igh distal enhancer modulates antigen receptor diversity by determining locus conformation
    Authors: KH Bhat, S Priyadarsh, S Naiyer, X Qu, H Farooq, E Kleiman, J Xu, X Lei, JF Cantillo, R Wuerffel, N Baumgarth, J Liang, AJ Feeney, AL Kenter
    Nature Communications, 2023;14(1):1225.  2023
  3. Loop extrusion promotes an alternate pathway for isotype switching
    Authors: HM Shen, R Wuerffel, JF Cantillo, S Priyadarsh, X Lei, J Liang, YL Wu, AL Kenter
    Cell Reports, 2021-11-23;37(8):110059.  2021-11-23
  4. Foxo3 Promotes the Differentiation and Function of Follicular Helper T Cells
    Authors: H Qi, D Tian, M Li, C Zhang, H Jin, L Liu, X Zhao, L Ma, W Zhao, Q Ge, T Duan, D Zhang
    Cell Rep, 2020-05-12;31(6):107621.  2020-05-12
  5. TAGLN2 polymerizes G-actin in a low ionic state but blocks Arp2/3-nucleated actin branching in physiological conditions
    Authors: HR Kim, MS Kwon, S Lee, Y Mun, KS Lee, CH Kim, BR Na, BNR Kim, I Piragyte, HS Lee, Y Jun, MS Jin, YM Hyun, HS Jung, JY Mun, CD Jun
    Sci Rep, 2018-04-03;8(1):5503.  2018-04-03
  6. Cytokine- and TCR-Mediated Regulation of T Cell Expression of Ly6C and Sca-1
    Authors: JH DeLong, AO Hall, C Konradt, GM Coppock, J Park, G Harms Prit, CA Hunter
    J. Immunol., 2018-01-22;0(0):.  2018-01-22
  7. Lineage-Specific Metabolic Properties and Vulnerabilities of T Cells in the Demyelinating Central Nervous System
    Authors: SM Seki, M Stevenson, AM Rosen, S Arandjelov, L Gemta, TNJ Bullock, A Gaultier
    J. Immunol., 2017-05-15;0(0):.  2017-05-15
  8. Concomitant Disruption of CD4 and CD8 Genes Facilitates the Development of Double Negative ?? TCR(+) Peripheral T Cells That Respond Robustly to Staphylococcal Superantigen
    Authors: VR Chowdhary, A Krogman, AY Tilahun, MP Alexander, CS David, G Rajagopala
    J. Immunol., 2017-05-03;0(0):.  2017-05-03
  9. IL-12 conditioning improves retrovirally mediated transduction efficiency of CD8+ T cells.
    Authors: Andrijauskaite K, Suriano S, Cloud C, Li M, Kesarwani P, Stefanik L, Moxley K, Salem M, Garrett-Mayer E, Paulos C, Mehrotra S, Kochenderfer J, Cole D, Rubinstein M
    Cancer Gene Ther, 2015-07-17;22(7):360-7.  2015-07-17
  10. TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse.
    Authors: Na B, Kim H, Piragyte I, Oh H, Kwon M, Akber U, Lee H, Park D, Song W, Park Z, Im S, Rho M, Hyun Y, Kim M, Jun C
    J Cell Biol, 2015-04-13;209(1):143-62.  2015-04-13
  11. Accumulation of 4-1BBL+ B cells in the elderly induces the generation of granzyme-B+ CD8+ T cells with potential antitumor activity.
    Authors: Lee-Chang, Catalina, Bodogai, Monica, Moritoh, Kanako, Olkhanud, Purevdor, Chan, Andrew C, Croft, Michael, Mattison, Julie A, Holst, Peter Jo, Gress, Ronald E, Ferrucci, Luigi, Hakim, Fran, Biragyn, Arya
    Blood, 2014-07-18;124(9):1450-9.  2014-07-18
  12. Soluble production of a biologically active single-chain antibody against murine PD-L1 in Escherichia coli.
    Authors: Drees J, Augustin L, Mertensotto M, Schottel J, Leonard A, Saltzman D
    Protein Expr Purif, 2013-11-08;94(0):60-6.  2013-11-08
  13. Efficacy and mechanism-of-action of a novel superagonist interleukin-15: interleukin-15 receptor alphaSu/Fc fusion complex in syngeneic murine models of multiple myeloma.
    Authors: Xu W, Jones M, Liu B, Zhu X, Johnson C, Edwards A, Kong L, Jeng E, Han K, Marcus W, Rubinstein M, Rhode P, Wong H
    Cancer Res, 2013-05-03;73(10):3075-86.  2013-05-03
  14. A role for IL-27p28 as an antagonist of gp130-mediated signaling.
    Authors: Stumhofer JS, Tait ED, Quinn WJ, Hosken N, Spudy B, Goenka R, Fielding CA, O'Hara AC, Chen Y, Jones ML, Saris CJ, Rose-John S, Cua DJ, Jones SA, Elloso MM, Grotzinger J, Cancro MP, Levin SD, Hunter CA
    Nat. Immunol., 2010-11-07;11(12):1119-26.  2010-11-07
  15. Loss of AMPK exacerbates experimental autoimmune encephalomyelitis disease severity.
    Authors: Nath N, Khan M, Rattan R, Mangalam A, Makkar RS, de Meester C, Bertrand L, Singh I, Chen Y, Viollet B, Giri S
    Biochem. Biophys. Res. Commun., 2009-05-30;386(1):16-20.  2009-05-30
  16. IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response.
    Authors: Rubinstein MP, Lind NA, Purton JF, Filippou P, Best JA, McGhee PA, Surh CD, Goldrath AW
    Blood, 2008-08-08;112(9):3704-12.  2008-08-08
  17. TIM-4 expressed on APCs induces T cell expansion and survival.
    Authors: Rodriguez-Manzanet R, Meyers JH, Balasubramanian S, Slavik J, Kassam N, Dardalhon V, Greenfield EA, Anderson AC, Sobel RA, Hafler DA, Strom TB, Kuchroo VK
    J. Immunol., 2008-04-01;180(7):4706-13.  2008-04-01
  18. Simultaneous deletion of MyD88 and Trif delays major histocompatibility and minor antigen mismatch allograft rejection.
    Authors: McKay D, Shigeoka A, Rubinstein M, Surh C, Sprent J
    Eur. J. Immunol., 2006-08-01;36(8):1994-2002.  2006-08-01

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