Home » Ephrin-A4 » Ephrin-A4 Lysates » Ephrin-A4 Overexpression Lysate

Ephrin-A4 Overexpression Lysate

 Submit a Review

Images

 
•
(
Enlarge)
Western Blot: Ephrin A4 Overexpression Lysate (Adult Normal) [NBP2-10484] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ephrin A4.
Western Blot: Ephrin A4 Overexpression Lysate (Adult Normal) [NBP2-10484] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ephrin A4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

  • Loading...
Novus Biologicals offers 100% Guarantee
Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Ephrin-A4 Overexpression Lysate Summary

Description

Ephrin-A4 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC224388

Accession#: NM_005227

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
EFNA4

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
22.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Ephrin-A4 Overexpression Lysate

  • EFL4
  • EFL-4
  • EFNA4
  • EPH-related receptor tyrosine kinase ligand 4
  • EphrinA4
  • Ephrin-A4
  • EPLG4MGC125826
  • LERK-4
  • LERK4FLJ57652
  • ligand of eph-related kinase 4

Background

The Eph subfamily represents the largest group of receptor protein kinases identified to date. There is increasing evidence that Eph family members are involved in central nervous system function and in development. Ligands for Eph receptors include ephrin-A1 (LERK-1/B61), identified as a ligand for the EphA2 (Eck) receptor; ephrin-A2 (ELF-1), identified as a ligand for the EphA3 and EphA4 (Sek) receptors; ephrin-A3 (LERK-3), identified as a ligand for EphA5 (Ehk1) and EphA3 (Hek) receptors; ephrin-A4 (LERK-4), identified as a ligand for the EphA3 receptor; ephrin-A5 (AL-1), identified as a ligand for EphA5 (REK7); ephrin-B1 (LERK-2), identified as a ligand for the EphB1 (Elk) and EphB2 (Cek5) receptors; ephrin-B2 (LERK-5), identified as a ligand for the EphB1, EphB3 (Cek10) and EphB2 receptors; and ephrin-B3 (LERK-8), identified as a ligand for EphB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Ephrin-A3
[Unconjugated]
359-EA
Ephrin-A3 [Unconjugated]
(10 Publications)
Species: Hu
Applications: Bind
Ephrin-A1
602-A1
Ephrin-A1
(20 Publications)
Species: Mu
Applications: Bind

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Ephrin-A4 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol EFNA4