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ELOVL6 Overexpression Lysate

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-06354
    • Availability
      Product Discontinued

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ELOVL6 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_076995. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ELOVL6, mRNA.
Gene
ELOVL6

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ELOVL6 Overexpression Lysate

  • elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast)
  • ELOVL fatty acid elongase 6,3-keto acyl-CoA synthase ELOVL6
  • FAE
  • fatty acid elongase 2
  • long-chain fatty-acyl elongase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs

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Bioinformatics

Gene Symbol ELOVL6