MAN2B1 293T Cell Transient Overexpression Lysate

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Summary
Product Discontinued
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    • Catalog Number
      H00004125-T01
    • Availability
      Product Discontinued

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MAN2B1 293T Cell Transient Overexpression Lysate Summary

Description
Quality control test: Transient overexpression cell lysate was tested with Anti-MAN2B1 antibody by Western Blots. Plasmid: pCMV-MAN2B1 full-length
Specificity
MAN2B1 293T Cell Transient Overexpression Lysate(Denatured)
Gene
MAN2B1

Applications/Dilutions

Dilutions
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

Type
293T Cell Transient Overexpression
Protein State
Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAN2B1 293T Cell Transient Overexpression Lysate

  • alpha B, lysosomal
  • EC 3.2.1
  • EC 3.2.1.24
  • LAMAN
  • Lysosomal acid alpha-mannosidase
  • MAN2B1
  • MANB
  • Mannosidase alpha class 2B member 1
  • Mannosidase Alpha-B
  • mannosidase, alpha, class 2B, member 1

Background

MAN2B1 encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MAN2B1