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PU.1/Spi-1 Overexpression Lysate (Denatured)

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Summary
Product Discontinued
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    • Catalog Number
      H00006688-T02
    • Availability
      Product Discontinued

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PU.1/Spi-1 Overexpression Lysate (Denatured) Summary

Description
Quality control test: Transient overexpression cell lysate was tested with Anti-SPI1 antibody by Western Blots. Plasmid: pCMV-SPI1 full-length
Gene
SPI1

Applications/Dilutions

Dilutions
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for Array

Type
Overexpression
Protein State
Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PU.1/Spi-1 Overexpression Lysate (Denatured)

  • hematopoietic transcription factor PU.1
  • OF
  • PU.1
  • SFPI1
  • SFPI131 kDa transforming protein
  • SPI-1 proto-oncogene
  • SPI1
  • Spi-1
  • SPI-A
  • spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein
  • transcription factor PU.1

Background

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SPI1