GCAP1 Overexpression Lysate

Images

 
Western Blot: GCAP1 Overexpression Lysate (Adult Normal) [NBL1-11406] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GCAP1.
Western Blot: GCAP1 Overexpression Lysate (Adult Normal) [NBL1-11406] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for GUCA1A

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GCAP1 Overexpression Lysate Summary

Description

GCAP1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC206595

Accession#: NM_000409

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
GUCA1A

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for GCAP1 Overexpression Lysate

  • C6orf131
  • chromosome 6 open reading frame 131
  • CORD14
  • GCAP 1
  • GCAP1photoreceptor 1
  • GCAPGUCA
  • guanylate cyclase activator 1A (retina)
  • Guanylate cyclase activator 1A
  • guanylin 1, retina
  • guanylyl cyclase-activating protein 1
  • GUCA1

Background

This gene plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein encoded by this gene, guanylyl cyclase activating protein 1 (GCAP1), mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP1 promotes activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in this gene cause autosomal dominant cone dystrophy (COD3); a disease characterized by reduced visual acuity associated with progressive loss of color vision. Mutations in this gene prohibit the inactivation of RetGC1 at high Ca(2+) concentrations; causing the constitutive activation of RetGC1 and, presumably, increased cell death. This gene is expressed in retina and spermatagonia. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-68721
Species: Hu, Pm
Applications: IHC, IHC-Fr, IHC-P, WB
NBP3-12211
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP3-38258
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
664-LI
Species: Hu
Applications: BA
NBP3-12219
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-84880
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-94723
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, WB
NBP1-91930
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB110-3638
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
AF6386
Species: Hu
Applications: IP, WB
NBP1-84996
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00000251-M07
Species: Hu
Applications: ELISA, IHC, IHC-P, IP, KD, ELISA(Cap), S-ELISA, WB
NBP2-25160
Species: Bv, Ca, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, WB
NBP3-03109
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-60655
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89724
Species: Hu
Applications: IHC, IHC-P, WB
NBP3-17540
Species: Hu
Applications: ICC/IF, WB
NBP2-14871
Species: Mu, Rt
Applications: ICC/IF, WB
NB120-2860
Species: Ba, Bv, Ch, Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBL1-11406
Species: Hu
Applications: WB

Publications for GCAP1 Lysate (NBL1-11406) (0)

There are no publications for GCAP1 Lysate (NBL1-11406).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GCAP1 Lysate (NBL1-11406) (0)

There are no reviews for GCAP1 Lysate (NBL1-11406). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GCAP1 Lysate (NBL1-11406) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GCAP1 Products

Research Areas for GCAP1 Lysate (NBL1-11406)

Find related products by research area.

Blogs on GCAP1

There are no specific blogs for GCAP1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GCAP1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol GUCA1A