DAP Kinase 2 Overexpression Lysate

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBL1-09717
    • Availability
      Product Discontinued

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DAP Kinase 2 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC216274 and based on accession number NM_014326. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens death-associated protein kinase 2 (DAPK2), mRNA.
Gene
DAPK2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
42.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DAP Kinase 2 Overexpression Lysate

  • DAP Kinase 2
  • DAPK2
  • DAP-kinase-related protein 1 beta isoform
  • DAP-kinase-related protein 1
  • death-associated protein kinase 2
  • DRP1
  • DRP-1
  • DRP-1MGC119312
  • EC 2.7.11
  • EC 2.7.11.1

Background

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for DAP Kinase 2 Lysate (NBL1-09717) (0)

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Bioinformatics

Gene Symbol DAPK2