Home » ST3GAL4 » ST3GAL4 Lysates » ST3GAL4 Overexpression Lysate

ST3GAL4 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: ST3GAL4 Overexpression Lysate (Adult Normal) [NBP2-07648] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ST3GAL4.
Western Blot: ST3GAL4 Overexpression Lysate (Adult Normal) [NBP2-07648] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ST3GAL4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals offers 100% Guarantee

ST3GAL4 Overexpression Lysate Summary

Description

ST3GAL4 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC216090

Accession#: NM_006278

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
ST3GAL4

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
37.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for ST3GAL4 Overexpression Lysate

  • Alpha 2,3-sialyltransferase IV
  • Alpha 2,3-ST 4
  • Beta-galactoside alpha-2,3-sialyltransferase 4
  • CGS23
  • CGS23FLJ46764
  • EC 2.4.99
  • EC 2.4.99.-
  • EC 2.4.99.9
  • FLJ11867
  • gal-beta-1,4-GalNAc-alpha-2,3-sialyltransferase
  • Gal-NAc 6S
  • gal-NAc6S
  • NANTA3
  • NANTA3alpha-3-N-acetylneuraminyltransferase
  • SAT3
  • SAT-3
  • sialyltransferase 4C (beta-galactosidase alpha-2,3-sialytransferase)
  • sialyltransferase 4C (beta-galactoside alpha-2,3-sialytransferase)
  • Sialyltransferase 4C
  • SIAT4
  • SIAT4C
  • SIAT4-C
  • SIAT4CCMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4
  • ST3 beta-galactoside alpha-2,3-sialyltransferase 4
  • ST3Gal IV
  • ST3GAL4
  • ST3GalA.2
  • ST3GalIV
  • ST-4
  • STZSIAT4

Background

ST3GAL4 - ST3 beta-galactoside alpha-2,3-sialyltransferase 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(26 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Catalase Antibody
[Unconjugated]
AF3398
Western Blot Catalase Antibody [Unconjugated]
Simple Western Catalase Antibody [Unconjugated]
Knockout Validated Catalase Antibody [Unconjugated]
(3 Reviews)
(15 Publications)
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
SOD1/Cu-Zn SOD
Antibody - BSA Free
NBP2-24915
Western Blot SOD1/Cu-Zn SOD Antibody - BSA Free
Western Blot SOD1/Cu-Zn SOD Antibody - BSA Free
Immunohistochemistry-Paraffin SOD1/Cu-Zn SOD Antibody - BSA Free
(3 Reviews)
(21 Publications)
Species: Bv, Hu, Mu, Rt
Applications: IHC,  IHC-P, WB
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(57 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(828 Publications)
Species: Hu
Applications: BA
iNOS Antibody -
BSA Free
NB300-605
Western Blot iNOS Antibody - BSA Free
Western Blot iNOS Antibody - BSA Free
Western Blot iNOS Antibody - BSA Free
(3 Reviews)
(141 Publications)
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr,  IHC-P, In vitro, WB
Serpin
A8/Angiotensinogen An ...
NBP1-30027
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
Western Blot Serpin A8/Angiotensinogen Antibody - BSA Free
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
(12 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, WB
Sialin/SLC17A5
Antibody - BSA Free
NBP2-20383
Immunohistochemistry-Paraffin Sialin/SLC17A5 Antibody - BSA Free
Western Blot Sialin/SLC17A5 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Sialin/SLC17A5 Antibody - BSA Free
Species: Hu, Mu
Applications: ICC/IF, IHC,  IHC-P, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(689 Publications)
Species: Hu
Applications: ELISA
eNOS Antibody -
BSA Free
NB300-500
Western Blot eNOS Antibody - BSA Free
Immunohistochemistry-Paraffin eNOS Antibody - BSA Free
Immunohistochemistry-Paraffin eNOS Antibody - BSA Free
(3 Reviews)
(29 Publications)
Species: Am, Bv, Ca, Dr, Hu, Mu, Po, Rt, Sh
Applications: IHC, IHC-Fr,  IHC-P, WB
IGF-I/IGF-1
[Unconjugated]
291-G1
SDS-PAGE IGF-I/IGF-1 [Unconjugated]
Bioactivity IGF-I/IGF-1 [Unconjugated]
Mass Spectrometry IGF-I/IGF-1 [Unconjugated]
(229 Publications)
Species: Hu
Applications: BA
AKR1B1 Antibody -
BSA Free
NBP1-89146
Immunohistochemistry-Paraffin AKR1B1 Antibody - BSA Free
Immunohistochemistry-Paraffin AKR1B1 Antibody - BSA Free
Immunohistochemistry-Paraffin AKR1B1 Antibody - BSA Free
Species: Hu
Applications: ICC/IF, IHC,  IHC-P
DMPK Antibody
(713931)
MAB6967
Western Blot DMPK Antibody (713931)
Species: Hu
Applications: WB
nNOS Antibody
NB100-858
Immunocytochemistry/ Immunofluorescence nNOS Antibody
Flow Cytometry nNOS Antibody
Immunocytochemistry/ Immunofluorescence nNOS Antibody
(1 Review)
(15 Publications)
Species: Gp, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-WhMt, IHC, IHC-Fr,  IHC-P, PEP-ELISA, WB
RanGAP1 Antibody
(OTI1B4)
NBP2-02623
Flow Cytometry RanGAP1 Antibody (OTI1B4)
Flow Cytometry RanGAP1 Antibody (OTI1B4)
Flow Cytometry RanGAP1 Antibody (OTI1B4)
(1 Publication)
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC,  IHC-P, WB
Glucagon Antibody
(181402) [Unconjugate ...
MAB1249
Immunocytochemistry Glucagon Antibody (181402) [Unconjugated]
Intracellular Staining by Flow Cytometry Glucagon Antibody (181402) [Unconjugated]
(1 Review)
(16 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
ACE/CD143 Antibody
[Unconjugated]
AF1513
Immunohistochemistry ACE/CD143 Antibody [Unconjugated]
Western Blot ACE/CD143 Antibody [Unconjugated]
Simple Western ACE/CD143 Antibody [Unconjugated]
(1 Review)
(7 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
Thyroglobulin
Antibody (2H11 + 6E1)
NBP2-34294
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
(3 Reviews)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, IHC,  IHC-P
Glut4 Antibody -
BSA Free
NBP1-49533
Flow (Intracellular) Glut4 Antibody - BSA Free
Western Blot Glut4 Antibody - BSA Free
Western Blot Glut4 Antibody - BSA Free
(4 Reviews)
(43 Publications)
Species: Hu, Mu, Pl, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC,  IHC-P, KD, WB
GLYAT Antibody -
BSA Free
NBP2-58917
Western Blot GLYAT Antibody - BSA Free
Immunohistochemistry-Paraffin GLYAT Antibody - BSA Free
Immunohistochemistry-Paraffin GLYAT Antibody - BSA Free
Species: Hu
Applications: IHC,  IHC-P, WB

Publications for ST3GAL4 Lysate (NBP2-07648) (0)

There are no publications for ST3GAL4 Lysate (NBP2-07648).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ST3GAL4 Lysate (NBP2-07648) (0)

There are no reviews for ST3GAL4 Lysate (NBP2-07648). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ST3GAL4 Lysate (NBP2-07648) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ST3GAL4 Products

Research Areas for ST3GAL4 Lysate (NBP2-07648)

Find related products by research area.

Blogs on ST3GAL4

There are no specific blogs for ST3GAL4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ST3GAL4 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol ST3GAL4