p53 (h3): 293T Lysate: sc-158802

p53 (m): 293T Lysate, derived from the HEK 293T cell line, is an invaluable resource for studying the tumor suppressor protein p53 in murine systems. The HEK 293T cells, a variant of the HEK 293 cells engineered to express the SV40 large T antigen, are optimized for high-efficiency protein expression, making this lysate ideal for detailed molecular and biochemical analyses of p53. Researchers utilize p53 (m): 293T Lysate to investigate the mechanisms by which p53 regulates cell cycle arrest, apoptosis, and DNA repair. p53 functions as a transcription factor that responds to various cellular stresses by activating or repressing the expression of target genes involved in maintaining genomic stability. Techniques such as chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSAs), and reporter gene assays are employed to study the DNA-binding activity of p53 and its interaction with co-factors and regulatory proteins. This lysate is instrumental in exploring the post-translational modifications of p53, such as phosphorylation and acetylation, which modulate its stability and activity. By analyzing these modifications, scientists gain insights into how p53 is regulated in response to cellular stress signals. Research using p53 (m): 293T Lysate focuses strictly on understanding the fundamental biological processes of p53's role in cell cycle control and apoptosis. This helps in advancing our knowledge of cellular mechanisms and the molecular dynamics of p53.