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Recombinant Human GST-AMSH-LP Protein, CF

Catalog #: E-551
1 Citation Datasheet / COA / SDS

Discontinued Product

E-551 has been discontinued.
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Recombinant Human GST-AMSH-LP Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Reaction conditions will need to be optimized for each specific application.  We recommend an initial human recombinant GST-AMSH-LP concentration of 100-500 nM when using K63-linked poly-Ubiquitin substrates.  AMSH-LP does not efficiently utilize Ubiquitin-AMC (U-550) as a substrate, though a signal may be obtained when the enzyme is used at a concentration of 500 nM or higher. 
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human AMSH-LP protein
Contains an N-terminal GST (glutathione S-transferase) tag
Accession #
Q96FJ0
Predicted Molecular Mass
79 kDa

Product Datasheets

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E-551

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-551

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Background: AMSH-LP

AMSH-like protease (AMSH-LP, also known as STAM-Binding Protein-Like 1, STAMBPL1) is a zinc metalloprotease belonging to the JAMM (JAB1/MPN/Mov34) family of deubiquitinating enzymes (DUBs). 436 amino acids in length, AMSH-LP is 56% identical and 73% similar to AMSH, an enzyme that functions at the endosome, where it is involved in the sorting of various cell-surface receptors to lysozomes. Like AMSH, AMSH-LP only cleaves K63-linked poly-Ubiquitin chains in vitro; however, unlike AMSH, AMSH-LP does not appear to associate with STAM protein. This recombinant protein is full-length and contains an N-terminal GST tag.

References
  1. Davies, C.W. et al. (2011) J. Mol. Biol.  413: 416
  2. Sato, Y. et al. (2008) Nature 455: 358
Long Name
STAM-binding Protein-like 1
Entrez Gene IDs
57559 (Human); 76630 (Mouse); 687696 (Rat)
Alternate Names
ALMalpha; ALMalpha;AMSH-FP;AMSH-like protease;AMSH-LP;bA399O19.2;STAMBPL1; AMSH-FP; AMSHLP; AMSH-LP; STAMBPL1

Citation for Recombinant Human GST-AMSH-LP Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Structural and functional characterization of ubiquitin variant inhibitors for the JAMM-family deubiquitinases STAMBP and STAMBPL1
    Authors: Y Guo, Q Liu, E Mallette, C Caba, F Hou, J Fux, G LaPlante, A Dong, Q Zhang, H Zheng, Y Tong, W Zhang
    The Journal of Biological Chemistry, 2021-08-21;0(0):101107.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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