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Recombinant Human M-CSF Protein

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SDS-Page: Recombinant Human M-CSF Protein [NBP2-34975]
SDS-Page: Recombinant Human M-CSF Protein [NBP2-34975]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier-Free
NBP2-34975-1mg
Carrier-Free
NBP2-34975-100ug
Carrier-Free
NBP2-34975-500ug
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Recombinant Human M-CSF Protein Summary

Description
A disulfide-linked homodimer consisting of two 158 amino acid polypeptide chains corresponding to M-CSF Source: E. coli

Uniprot ID: P09603-3

Amino Acid Sequence: EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQG HERQSEGS
Details of Functionality
MCSF Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CSF1
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of M-CSF as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
36.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS, pH7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water to a concentration of 0.1mg/mL.

Alternate Names for Recombinant Human M-CSF Protein

  • colony stimulating factor 1 (macrophage)
  • CSF1
  • CSF-1
  • Lanimostim
  • macrophage colony stimulating factor
  • macrophage colony-stimulating factor 1
  • MCSF
  • M-CSF
  • MCSFlanimostim
  • MGC31930

Background

Macrophage Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. M-CSF is a four alpha-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for M-CSF gene. Although forms may vary, all of them contain the N-terminal 150 a.a. portion that is necessary and sufficient for interaction with the receptor. The first 223 a.a. of mature human M-CSF shares 88 %, 86 %, 81 % and 74 % sequence identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CSF1