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Recombinant Human c-jun Protein

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Summary
Product Discontinued
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    • Catalog Number
      NBP1-99207
    • Availability
      Product Discontinued

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Recombinant Human c-jun Protein Summary

Description
Recombinant protein for Human Jun

Source:E. coli
Amino Acid Sequence:( P05412)
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
JUN
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Application Notes
Specific activity: C-Jun fusion protein at a concentration of 2 ug/20 ul reaction was phosphorylated in an in vitro kinase assay with 200 uM ATP. After 30 minutes incubation at 30C, phosphorylation was detected by Western blot with phospho-specific c-Jun antibodies.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 2 mM EDTA, 1 mM DTT, 50% glycerol
Concentration
2 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human c-jun Protein

  • Activator protein 1
  • AP1
  • AP-1
  • cJun
  • c-Jun
  • enhancer-binding protein AP1
  • Jun activation domain binding protein
  • jun oncogene
  • jun proto-oncogene
  • JUN
  • Proto-oncogene c-Jun
  • transcription factor AP-1
  • V-jun avian sarcoma virus 17 oncogene homologp39
  • v-jun sarcoma virus 17 oncogene homolog (avian)
  • v-jun sarcoma virus 17 oncogene homolog

Background

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins, and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. The MAP kinase homologue, JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. The activity of JNK is stimulated by the same signals that activates c-Jun.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Human
c-jun Protein
NBP1-99207
Recombinant Human c-jun Protein
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Applications: PAGE, Bioactivity

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Bioinformatics

Gene Symbol JUN