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BCL2L10 Antibody Blocking Peptide

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Western Blot: BCL2L10 Peptide [NBP1-77224PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C
Western Blot: BCL2L10 Peptide [NBP1-77224PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C

Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC
Concentration
0.2 mg/ml

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BCL2L10 Antibody Blocking Peptide Summary

Description
A blocking peptide containing 16 amino acids near the center of human LSD1.

Source: Synthetic

(Accession #: NP_065129)
Source
Synthetic
Protein/Peptide Type
Antibody Blocking Peptide
Gene
BCL2L10
Purity
N/A

Applications/Dilutions

Dilutions
  • Antibody Competition
Application Notes
This peptide is useful as a blocking peptide for NBP1-77224.

For further blocking peptide related protocol, click here.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
Preservative
0.02% Sodium Azide
Concentration
0.2 mg/ml
Purity
N/A

Alternate Names for BCL2L10 Antibody Blocking Peptide

  • Apoptosis regulator Bcl-B
  • apoptotic protein NrH
  • BCL2L10
  • Bcl2-L-10
  • BCL2-like 10 (apoptosis facilitator)
  • bcl-2-like protein 10
  • BCLB
  • BCL-B
  • BOO
  • DIVA
  • MGC129810
  • MGC129811

Background

The protein encoded by the BCL2L10 gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains conserved BH4, BH1 and BH2 domains. This protein can interact with other members of BCL-2 protein family including BCL2, BCL2L1/BCL-X(L), and BAX. Overexpression of this gene has been shown to suppress cell apoptosis possibly through the prevention of cytochrome C release from the mitochondria, and thus activating caspase-3 activation. The mouse counterpart of this protein is found to interact with Apaf1 and forms a protein complex with Caspase 9, which suggests the involvement of this protein in APAF1 and CASPASE 9 related apoptotic pathway. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for BID at approximately 29 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse BID Antigen Affinity-purified Polyclonal Antibody (Catalog # AF860) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
BID Antibody [Unconjugated]
AF860

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Bioinformatics

Gene Symbol BCL2L10