Why does interferon stand under 18 KD in SDS-PAGE gel?

Name: Recombinant Human IFN-alpha 2 Protein
Brand: Novus Biologicals
SKU: NBC1-18467
Price: 279 USD
Availability: Discontinued

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The molecular weight of 19.4 kDa for NBC1-18467 has been confirmed by MALDI-TOF. The migration and hence exact band position of a target protein in western blot can be influenced by many factors, such as different gel types and buffers. As the observed band is only slightly different from the theoretical molecular weight, I would think that this is the likely explanation. The lab have now replied to me in answer to your enquiry. They say, as I noted, that although size is the major determinant of band migration, other factors can affect it too to a certain extent (they give charge as another example). They also point out that the difference in this case is very small, and slight mis-alignment with the marker could be a cause. Finally, they emphasize that SDS-PAGE, MALDI-TOF and DNA sequence analysis of the protein has confirmed its composition.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Description	An un-tagged recombinant protein corresponding to the amino acids 24-188 of Human IFN-alpha 2 Source: E.coli Amino Acid Sequence: MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE
Source	E. coli
Protein/Peptide Type	Recombinant Protein
Gene	IFNA2
Purity	>95% pure by SDS-PAGE
Endotoxin Note	< 1.0 EU per 1 microgram of protein (determined by LAL method)

Dilutions	SDS-Page
Theoretical MW	19.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS (pH 7.4)
Preservative	No Preservative
Concentration	1 mg/ml
Purity	>95% pure by SDS-PAGE

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