Recombinant Human cleavage stimulation factor His Protein

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SDS-Page: cleavage stimulation factor Protein [NBP1-98871] - Analysis at 15% (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml
Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Recombinant Human cleavage stimulation factor His Protein Summary

Description
A denatured recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-431 of Human cleavage stimulation factor

Source: E.coli

Amino Acid Sequence: MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DVEHHHHHH

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CSTF1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Theoretical MW
49.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 2M UREA
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human cleavage stimulation factor His Protein

  • CF-1 50 kDa subunit
  • Cleavage stimulation factor 50 kDa subunit
  • cleavage stimulation factor subunit 1
  • cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kD
  • cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
  • CSTF 50 kDa subunit
  • CstF-50
  • CstFp50

Background

CSTF1, also known as cleavage stimulation factor subunit 1, is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. Recombinant human CSTF1 protein, fused to His-tag at C-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CSTF1