Recombinant Human RhoGDI His Protein

Images

 
SDS-Page: Recombinant Human RhoGDI His Protein [NBP1-50861] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human RhoGDI His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 24-204 of Human RhoGDI

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSVNYKPPAQ KSIQEIQELD KDDESLRKYK EALLGRVAVS ADPNVPNVVV TGLTLVCSSA PGPLELDLTG DLESFKKQSF VLKEGVEYRI KISFRVNREI VSGMKYIQHT YRKGVKIDKT DYMVGSYGPR AEEYEFLTPV EEAPKGMLAR GSYSIKSRFT DDDKTDHLSW EWNLTIKKDW KD

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ARHGDIA
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
22.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human RhoGDI His Protein

  • GDIA1
  • MGC117248
  • Rho GDI 1
  • Rho GDP dissociation inhibitor (GDI) alpha
  • rho GDP-dissociation inhibitor 1
  • Rho-GDI alpha
  • RHOGDI
  • RHOGDI-1

Background

Rho GDP-dissociation inhibitor 1, also known ARHGDIA, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. Localized to the cytoplasm, ARHGDIA inhibits the dissociation of GDP from Rho proteins, thereby preventing GTP from binding to and subsequently activating Rho proteins. In humans ARHGDIA can be phosphorylated at Ser 101 by p21-activated kinase, an event that inhibits ARHGDIA activity and may result in positive feedback regulation of certain ARHGDIA target proteins. Recombinant human ARHGDIA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol ARHGDIA