
Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling EIF2S2 using ab184549 at a 1/500 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution, counterstained with Dapi.

Western blot analysis of EIF2S2 in immunoprecipitation pellets from Jurkat lysate. ab184549 used at a 1/50 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution. Right lane shows PBS negative control.

Flow cytometric analysis of 2% paraformaldehyde fixed 293 cells labeling EIF2S2 using ab184549 at a 1/160 dilution (red) or a Rabbit monoclonal IgG control (green).Secondary antibody: Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution
![All lanes : Anti-EIF2S2 antibody [EPR15833(B)] (ab184549) at 1/20000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/12658_ab184549-217167-ab148549.jpg)
All lanes : Anti-EIF2S2 antibody [EPR15833(B)] (ab184549) at 1/20000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution